Differential actions of NPY on seizure modulation via Y1 and Y2 receptors: evidence from receptor knockout mice

Epilepsia
En-Ju D LinMatthew During

Abstract

Neuropeptide Y (NPY) has been shown to modulate seizure activities. To provide further understanding of the involvement of two of the most abundantly expressed NPY receptors, Y1 and Y2, we assessed the effect of Y1 and Y2 gene deletion on systemic kainic acid-induced seizures. We also examined the effect of rAAV-mediated hippocampal NPY overexpression on seizure susceptibility in these receptor knockout mice. Recombinant adeno-associated viral vector overexpressing NPY (rAAV-NPY) or an empty vector control (rAAV-Empty) was injected into the hippocampus of adult C57BL/6-129/SvJ wild-type male mice and mice deficient of Y1 or Y2 receptors on the same background. Four weeks after vector injection, mice were subjected to systemic kainic acid-induced seizures, and the seizure behaviors were scored. The rAAV-mediated hippocampal overexpression of NPY led to a twofold reduction in seizures induced by systemic kainic acid in wild-type mice and Y1 receptor knockout mice but not in mice deficient of Y2 receptors. A differential action by the receptors was observed in the seizure-induced mortality rate, with increased fatality in Y2-/- mice. In addition, although NPY overexpression did not significantly affect the mortality rate in Y2-/- ...Continue Reading

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Citations

Jan 30, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Andrei I MoloshAnantha Shekhar
May 28, 2008·Epilepsia·Fred A Lado, Solomon L Moshé
Aug 23, 2008·Epilepsia·Véronique RibanMatthew J During
Nov 14, 2012·Science Translational Medicine·Robert C WykesDimitri M Kullmann
Feb 6, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Veronica A LedouxCatherine S Woolley
Mar 19, 2014·Nature Reviews. Neurology·Dimitri M KullmannRobert C Wykes
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Sep 29, 2009·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·En-Ju Deborah LinHerbert Herzog
Nov 29, 2008·Experimental Neurology·Andreas T SørensenMerab Kokaia
Jul 26, 2008·Molecular and Cellular Neurosciences·Andreas T SørensenMerab Kokaia
Oct 31, 2012·Epilepsia·Andreas T Sørensen, Merab Kokaia
May 13, 2015·Neuropharmacology·Jeanelle PortelliIlse Smolders
Mar 31, 2015·Neurobiology of Disease·Kellen D WindenDan H Geschwind
Jul 1, 2014·Biochemical and Biophysical Research Communications·Noufissa KabliSusan R George
Dec 22, 2020·Frontiers in Molecular Neuroscience·Julia Alicja SzczygiełDavid Paul Drucker Woldbye
Jul 28, 2021·Molecular and Cellular Neurosciences·A PernaL Alberi Auber

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