Differential Activation of NLRP3, AIM2, and IFI16 Inflammasomes in Humans with Acute and Chronic Hepatitis B

Viral Immunology
Hongtao ChenShipin Wu

Abstract

Nod-like receptor protein 3 (NLRP3), absent in melanoma 2 (AIM2), and interferon gamma inducible protein 16 (IFI16) are innate immune sensors for intracellular microbes, which can be activated by various dangerous signals and subsequently lead to caspase-1 (CASP1) activation and the maturation cleavage of effector molecules pro-IL-1β and pro-IL-18. Their roles in immunopathology of acute and chronic hepatitis B virus (HBV) infection are still unclear. In this study, we first investigated the activation of NLRP3, AIM2, and IFI16 inflammasomes in peripheral blood mononuclear cells (PBMCs) from patients infected with acute hepatitis B (AHB) and chronic hepatitis B (CHB) by quantitative real-time PCR and enzyme-linked immunosorbent assay. We next analyzed the impact of hepatitis B e antigen (HBeAg) on activation of AIM2 and IFI16 inflammasomes in PBMCs of CHB patients stimulated in vitro with AIM2 and IFI16 agonist ligands, poly (dA:dT) and VACA-70mer, respectively. The results showed that the mRNA expression levels of AIM2, IFI16, and CASP1 in PBMCs from AHB and CHB patients were both upregulated. Furthermore, the mRNA levels of AIM2 and IFI16 in CHB patients were significantly positively correlated with serum HBV loads. However, ...Continue Reading

Associated Clinical Trials

References

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Dec 31, 2003·Lancet·Ching Lung LaiThierry Poynard
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Citations

Aug 3, 2019·European Journal of Immunology·Bhesh Raj SharmaThirumala-Devi Kanneganti
Sep 11, 2020·International Journal of Molecular Sciences·Beatriz Lozano-Ruiz, José M González-Navajas
Oct 13, 2020·Frontiers in Cellular and Infection Microbiology·Austin M Jeffries, Ian Marriott
Nov 24, 2019·Life Sciences·Ramesh Chandra Rai
Apr 17, 2021·Computational and Mathematical Methods in Medicine·Conghui ZhangXinghui Liu

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Methods Mentioned

BETA
PCR
PMA
enzyme-linked immunosorbent assay
ELISA
enzyme-linked
transfection

Software Mentioned

GraphPad

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