Differential activation of signaling pathways by UVA and UVB radiation in normal human epidermal keratinocytes.

Photochemistry and Photobiology
Deeba SyedHasan Mukhtar

Abstract

Ultraviolet (UV) radiation from the solar spectrum is a major etiological factor for many cutaneous pathologies including cancer. By understanding changes in cell signaling pathways induced by UVA and UVB, novel strategies for prevention and treatment of UV-related pathologies could be developed. However, much of the information in the literature from various laboratories cannot cross talk because of difficulties associated with the use of ill-defined light sources and physiologically irrelevant light dosimetry. Herein, we have assessed the effect of exposure of normal human epidermal keratinocytes (NHEK) to UVA (2 and 4 J cm(-2)) or UVB (20 and 40 mJ cm(-2)) radiation. Employing western blot analysis, we found that exposure of NHEK to UVB, but not UVA, phosphorylates JNK1/2 at Th(183)/Tyr(185), STAT3 at Ser(727) , AKT at Ser(473) and increases c-Fos expression, whereas exposure to UVA, but not UVB, phosphorylates AKT at Thr(308). UVB as well as UVA exposure leads to increased phosphorylation of (1) ERK1/2 at Th(202)/Tyr(204); (2) p38 at Th(180)/Tyr(204); (3) STAT3 at Tyr(705); (4) mTOR at Thr(2448); and (v) p70S6k at Thr(421) /Ser(424); enhanced expression of PI3K (p85) and c-jun; and nuclear translocation of NFκB proteins. Th...Continue Reading

References

Sep 1, 1979·Journal of the American Academy of Dermatology·K H KaidbeyA M Kligman
Apr 27, 2001·Journal of Dermatological Science·V ChaturvediB J Nickoloff
Sep 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Katrin RamsauerPavel Kovarik
Sep 20, 2002·Skin Pharmacology and Applied Skin Physiology·H Sánchez RuderischB Tebbe
Sep 20, 2002·Skin Pharmacology and Applied Skin Physiology·Jing ShangB Tebbe
Oct 20, 2004·The British Journal of Dermatology·P J HamptonJ J Lloyd
Apr 28, 2005·Experimental Dermatology·Steen LisbyHans Christian Wulf
Aug 2, 2006·Mutation Research·Mariarosaria D'ErricoEugenia Dogliotti
Aug 3, 2006·Experimental Dermatology·Farrukh Afaq, Hasan Mukhtar
May 15, 2007·The Journal of Investigative Dermatology·Christine JeanGuy Laurent
Aug 22, 2008·The Journal of Biological Chemistry·Cong CaoYinsheng Wan

❮ Previous
Next ❯

Citations

Jan 15, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Hiromi MuraseHideaki Hara
Jun 29, 2014·Archives of Dermatological Research·Michael S Leo, Raja K Sivamani
Dec 20, 2013·The Journal of Investigative Dermatology·Arnaud GalichetEliane J Müller
Nov 26, 2014·Environmental and Molecular Mutagenesis·Eva SedlačkováAlena Gábelová
Sep 5, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Rossella CalòLaura Marabini
Jun 28, 2015·Journal of Translational Medicine·Bodo C Melnik
May 5, 2016·Journal of Plastic Surgery and Hand Surgery·Byung-Joon JeonNa-Hyun Hwang
Oct 30, 2015·Cell Biology International·Haeyoung KwonSeyeon Park
Nov 17, 2016·The Journal of Investigative Dermatology·Kazuyuki Yo, Thomas M Rünger
Jan 23, 2017·Free Radical Biology & Medicine·André Passaglia SchuchCamila Carrião Machado Garcia
Jan 8, 2017·Photochemistry and Photobiology·Mary K Montes de OcaFarrukh Afaq
Mar 8, 2014·Acta Cirúrgica Brasileira·Juliana Pedrosa KorinfskyCeleste Maria
Jun 9, 2018·International Journal of Molecular Sciences·Cristina M NiculiţeMircea Leabu
Aug 14, 2018·Photochemistry and Photobiology·Gagan ChhabraNihal Ahmad
Jan 17, 2015·Biomolecules & Therapeutics·Ok-Nam BaeTae Cheon Jeong
Sep 30, 2016·The Biochemical Journal·Robert P Feehan, Lisa M Shantz
Apr 30, 2020·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·G GorgisenM C Ragbetli
Aug 11, 2020·Critical Reviews in Food Science and Nutrition·David Fonseca HernandezLuis Mojica
Aug 16, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Ken ShiratoTakako Kizaki
Jan 24, 2016·Cancer Prevention Research·Sally E DickinsonG Timothy Bowden
Jul 13, 2021·The Journal of Investigative Dermatology·Anngela C AdamsKaren Taraszka Hastings
Aug 20, 2021·Environmental Science and Pollution Research International·Keshav GoyalSandeep Mittan

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.