PMID: 9651499Jul 4, 1998Paper

Differential activation of system A and betaine/GABA transport in MDCK cell membranes by hypertonic stress

Biochimica Et Biophysica Acta
S A Kempson

Abstract

Accumulation of osmolytes by renal cells is due in part to increased uptake via specific transporters. These include amino acid transport system A and the betaine/GABA transporter (BGT1). Transport changes have been characterized using intact cells which makes the intracellular mechanisms difficult to determine. In this study the hypertonic upregulation of system A and BGT1 was studied directly at the membrane level in Madin-Darby canine kidney (MDCK) cells. Both system A and BGT1 transport systems were detected in an isolated membrane fraction containing plasma membranes. System A transport was increased in membranes prepared from cells after 6 h hypertonic stress (449 mosmol/kg) but BGT1 activity was minimal and not different from isotonic controls. The increase in system A was blocked by inhibitors of RNA and protein synthesis. BGT1 transport was induced in membranes prepared after 24 h hypertonicity. At this time system A activity in the membrane fraction remained increased, unlike the downregulation observed in intact MDCK cells. We conclude that differential upregulation of system A and BGT1 by hypertonic stress is due to intrinsic changes in these transporters at the membrane level. In contrast, the downregulation of sys...Continue Reading

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Citations

Apr 27, 2001·Biochemical and Biophysical Research Communications·R R AlfieriK P Wheeler
Dec 23, 2006·Pflügers Archiv : European journal of physiology·Xue-Mei ZhangSandra E Guggino
Feb 27, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·H KettunenM Saarinen
May 31, 2001·Kidney International·J C BashamS A Kempson
Feb 16, 2005·Annual Review of Physiology·Wolfgang Neuhofer, Franz-X Beck
May 2, 2008·American Journal of Physiology. Renal Physiology·Stephen A KempsonMichael Sturek
Jun 10, 2005·Cell Biochemistry and Function·Philip E LammersStephen A Kempson
Jun 14, 2005·Biochimica Et Biophysica Acta·Stephen A KempsonMarshall H Montrose
Mar 13, 2012·Biochemical and Biophysical Research Communications·Zohreh HosseinzadehFlorian Lang
May 6, 2014·Frontiers in Physiology·Stephen A KempsonNiels C Danbolt
Jun 9, 2001·Molecular Membrane Biology·O BussolatiG C Gazzola
Mar 10, 2006·American Journal of Physiology. Renal Physiology·Stephen A KempsonMichael Sturek
Feb 24, 2012·AJR. American Journal of Roentgenology·Madhavi PatnanaChristine O Menias
Jul 4, 2003·American Journal of Physiology. Cell Physiology·Stephen A KempsonMarshall H Montrose
Jul 25, 2008·American Journal of Physiology. Cell Physiology·Pierre BissonnetteJean-Yves Lapointe
Aug 6, 2009·The American Journal of Surgical Pathology·Ricardo S MacarencoAndre M Oliveira
Oct 26, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·P G PetroniniK P Wheeler
Jan 16, 2003·American Journal of Physiology. Renal Physiology·Jeremy L BrickerStephen A Kempson
Sep 9, 2015·The Biochemical Journal·Eva S SchweikhardChristine Ziegler

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