Differential activation of vascular genes by hypoxia in primary endothelial cells

Experimental Cell Research
Ingrid NilssonStefan Wennström

Abstract

Changes in the local environment, such as reduced oxygen tension (hypoxia), elicit transcriptional activation of a variety of genes in mammalian cells. Here we have analyzed the effect of hypoxia in different vascular endothelial cells (ECs) with emphasis on hypoxia-regulated transcription factors and genes of importance for blood vessel dynamics. While hypoxia induced the transcription factor hypoxia-inducible factor-1alpha (HIF-1alpha) in all endothelial cells tested, the closely related HIF-2alpha protein was markedly induced in microvascular/capillary endothelial cells, but only weakly or not at all in artery and vein endothelial cells. Furthermore, microvascular/capillary endothelial cells responded to hypoxia with increased number of transcripts encoding vascular endothelial growth factor-A (VEGF-A), VEGF receptor-2, the angiopoietin receptor Tie2, platelet-derived growth factor-B (PDGF-B), and inducible nitric oxide synthase (iNOS). In vein endothelial cells, hypoxia instead increased transcripts encoding lymphatic vascular components VEGF-C, -D, and VEGF receptor-3. Finally, reduced VEGF receptor levels and phosphorylation indicated establishment of a functional autocrine VEGF-A loop in hypoxic endothelial cells. Our re...Continue Reading

References

Jan 1, 1991·Pharmacology & Therapeutics·M D Randall
Sep 1, 1991·The Journal of Clinical Investigation·S KourembanasD V Faller
Dec 29, 1995·The Journal of Biological Chemistry·A NamikiJ M Isner
Aug 14, 1996·Biochemical and Biophysical Research Communications·C M WienerG L Semenza
Sep 1, 1996·Molecular and Cellular Biology·J A ForsytheG L Semenza
Nov 20, 1997·Journal of Cellular Physiology·I FlammeW Risau
Aug 5, 1998·Pharmacology & Therapeutics·E Thorin, S M Shreeve
Oct 29, 1998·The Journal of Biological Chemistry·B Q ShenT F Zioncheck
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·H H Marti, W Risau
Feb 23, 1999·Clinical and Experimental Pharmacology & Physiology·D V Faller
Apr 26, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·C HornigH A Weich
Jun 6, 2000·Biochimica Et Biophysica Acta·C MichielsJ Remacle
Jul 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·J PengG H Fong
Feb 13, 2001·Cardiovascular Research·E M ConwayP Carmeliet
Aug 23, 2001·Trends in Molecular Medicine·G L Semenza
Oct 24, 2001·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yukio NiimiKenji Fujiwara
Dec 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J FavierJ M Gasc
Jun 28, 2002·Physiological Reviews·Lotta Jussila, Kari Alitalo
Dec 20, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Michael S WiesenerKai-Uwe Eckardt
Apr 22, 2003·Journal of Cellular Physiology·William Durante
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter

❮ Previous
Next ❯

Citations

Jun 8, 2014·Domestic Animal Endocrinology·G BasiniF Grasselli
Jun 17, 2014·Critical Reviews in Oncology/hematology·Jennifer C BoerWilfred F A den Dunnen
Nov 23, 2006·Journal of Insect Science·Preeyada KoywiwattrakulRyszard Maleszka
Jul 19, 2013·Journal of Crohn's & Colitis·Pablo M LinaresJavier P Gisbert
Apr 16, 2005·Neuro-oncology·Balveen KaurErwin G Van Meir
Mar 28, 2007·Thorax·Alan J KnoxRachel Clifford
May 13, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Katherine A RadekLuisa A DiPietro
Mar 31, 2011·World Journal of Gastroenterology : WJG·Bin DuXu-Long Zhou
Jun 10, 2011·American Journal of Physiology. Cell Physiology·Stacy M ButlerWilliam J Pearce
Feb 5, 2013·American Journal of Physiology. Heart and Circulatory Physiology·P I ImoukhuedeAleksander S Popel
Mar 5, 2013·Physiology·Carolina HagbergUlf Eriksson
Mar 24, 2006·Breast Cancer Research and Treatment·Sebastian F SchoppmannPeter Birner
Jan 11, 2012·Antioxidants & Redox Signaling·Sanja CosoStavros Selemidis
Nov 7, 2007·Clinical & Experimental Metastasis·Suzanne EcclesJonathan Sleeman
May 21, 2008·Cardiovascular Research·Cora M L BeckersGeerten P van Nieuw Amerongen
Dec 29, 2005·Lymphatic Research and Biology·Mark E KleinmanGeoffrey C Gurtner
Apr 28, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Massimo Dal MontePaola Bagnoli
Jan 16, 2007·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Jing TaoChunyou Wang
Dec 25, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Shunqing ZuoJialong Guo
Jul 31, 2012·FEBS Letters·Idit ShacharShirly Becker-Herman
Nov 6, 2007·Trends in Immunology·Michele De PalmaClaire E Lewis
Aug 1, 2007·Journal of Thrombosis and Haemostasis : JTH·W C Aird
Apr 7, 2006·The Journal of Pharmacy and Pharmacology·Sherif Y SaadMaha M Arafah
Dec 25, 2007·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Pin-Yi LinChun-Pin Chiang
Jan 1, 2013·BioMed Research International·Esther RaskopfVolker Schmitz
Mar 31, 2015·The Cancer Journal·Valéry L PayenPierre Sonveaux
Oct 15, 2013·European Journal of Pharmacology·Masoumeh E GiviEsmaeil Mortaz
Sep 13, 2016·Auris, Nasus, Larynx·Mitsuharu AgaTomokazu Yoshizaki
Jul 4, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Scott A GerberJordan S Pober

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