Differential activity of progesterone and glucocorticoid receptors on mouse mammary tumor virus templates differing in chromatin structure.

The Journal of Biological Chemistry
C L SmithG L Hager

Abstract

In vivo, transcription factors interact with promoters having complex nucleoprotein structures. The transiently expressed progesterone receptor (PR) efficiently activates a transfected mouse mammary tumor virus (MMTV) promoter but is a poor activator of the MMTV promoter when it acquires an ordered chromatin structure as an endogenous, replicating gene. We show that the deficiency in PR activity is not due to insufficient expression of either B or A isoforms or competition between the two types of MMTV templates. Rather, this deficiency reflects an inability to induce the chromatin remodeling event that is required for activation of the replicated MMTV template. To determine whether this characteristic is common to transiently expressed steroid receptors or specific to the PR, we examined the activity of transiently expressed glucocorticoid (GR) receptor. Unlike the PR, the transiently expressed GR is an effective activator of both MMTV templates and efficiently induces the necessary chromatin remodeling event at the replicated template. These results indicate that the GR and PR have unique requirements for activation of promoters with ordered chromatin structure. These differences may provide a mechanism for establishing targe...Continue Reading

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Citations

Mar 1, 2006·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Gordon L HagerSam John
Jul 3, 2003·Experimental Cell Research·Niveen M MulhollandCatharine L Smith
Aug 12, 1998·The Journal of Steroid Biochemistry and Molecular Biology·G L HagerH Htun
Oct 16, 2004·Molecular and Cellular Biology·Joëlle RüeggTheo Rein
Oct 22, 2008·The International Journal of Biochemistry & Cell Biology·Anuja A GeorgeGordon L Hager
Sep 16, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Elisabeth D MartinezGordon L Hager
Feb 14, 1998·The Journal of Biological Chemistry·C J FryerT K Archer
Jan 28, 2004·The Journal of Biological Chemistry·Jeannine BotosCatharine L Smith
Jul 12, 2007·The Journal of Biological Chemistry·Monika LichtingerMichael Rehli
Nov 5, 1997·The Journal of Biological Chemistry·C L Smith, G L Hager

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