Differential activity of the FGF-4 enhancer in F9 and P19 embryonal carcinoma cells

Journal of Cellular Physiology
Brian BoerAngie Rizzino

Abstract

Transcription factors Oct-3/4 and Sox2 behave as global regulators during mammalian embryogenesis. They work together by binding co-operatively to closely spaced HMG and POU motifs (HMG/POU cassettes). Recently, it was suggested that a critical Sox2:Oct-3/4 target gene, FGF-4, is expressed at lower levels in P19 than in F9 embryonal carcinoma (EC) cells, due to lower levels of Sox2 in P19 than in F9 cells. We tested this possibility to better understand how FGF-4 expression is modulated during development. Although we found that P19 EC cells express approximately 10-fold less FGF-4 mRNA than F9 EC cells, we determined that Sox2 levels do not differ markedly in F9 and P19 EC cells. We also determined that Sox2 and Oct-3/4 work together equally well in both EC cell lines. Moreover, in contrast to an earlier prediction based on in vitro binding studies, we demonstrate that the function of the HMG/POU cassettes of the FGF-4 and UTF1 genes does not differ significantly in these EC cell lines when tested in the context of a natural enhancer. Importantly, we determined that the FGF-4 promoter is highly responsive to a heterologous enhancer in both EC cell lines; whereas, the FGF-4 enhancer is 7- to 10-fold less active in P19 than in F...Continue Reading

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Citations

Sep 30, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Harini ChakravarthyAngie Rizzino
Nov 3, 2011·PloS One·Hai-yong ZhaoYu-fei Shen
Dec 18, 2009·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Angie Rizzino
Mar 18, 2011·Journal of Cellular Physiology·Sunil K Mallanna, Angie Rizzino
Jan 24, 2008·Molecular Reproduction and Development·Sunil Kumar MallannaAngie Rizzino
Apr 5, 2017·Stem Cells International·Gregory M Kelly, Mohamed I Gatie

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