Abstract
The integrity of the alveolar epithelium is a key factor in the outcome of acute lung injury. Here, we investigate alveolar epithelial injury and the expression of epithelial-selective markers in Streptococcus pneumoniae-induced acute lung injury. S. pneumoniae was instilled into rat lungs and alveolar type I (RTI(40)/podoplanin, MMC6 antigen) and alveolar type II (MMC4 antigen, surfactant protein D, pro-surfactant protein C, RTII(70)) cell markers were quantified in lavage fluid and lung tissue at 24 and 72 hours. The alveolar epithelium was also examined using electron, confocal, and light microscopy. S. pneumoniae induced an acute inflammatory response as assessed by increased total protein, SP-D, and neutrophils in lavage fluid. Biochemical and morphological studies demonstrated morphologic injury to type II cells but not type I cells. In particular, the expression of RTI(40)/podoplanin was dramatically reduced, on the surface of type I cells, in the absence of morphologic injury. These data demonstrate that type II cell damage can occur in the absence of type I cell injury without affecting the ability of the lung to return to a normal morphology. These data also demonstrate that RTI(40)/podoplanin is not a type I cell phe...Continue Reading
References
Oct 1, 1992·American Journal of Respiratory Cell and Molecular Biology·M F BeersA B Fisher
Nov 1, 1989·The Journal of Pathology·G C RhodesL W Smith
Apr 1, 1989·The Journal of Pathology·G C RhodesA W Lykke
Jul 1, 1989·Infection and Immunity·A M BerryJ C Paton
Jun 30, 1988·Biochimica Et Biophysica Acta·L G DobbsR Gonzalez
May 11, 1995·The New England Journal of Medicine·E I TuomanenH R Masure
Oct 1, 1993·Infection and Immunity·P G DuaneE N Janoff
Apr 1, 1993·Infection and Immunity·J B RubinsD E Niewoehner
Feb 1, 1996·Bone·A WetterwaldM Atkinson
Aug 1, 1997·The American Journal of Physiology·L M Coluccio
Nov 14, 1998·Biochimica Et Biophysica Acta·E C Crouch
Jan 7, 1999·The European Respiratory Journal·R KoslowskiM Kasper
Sep 24, 1999·Thorax·J R Catterall
Sep 25, 1999·Infection and Immunity·M C McElroyT J Foster
May 4, 2000·The New England Journal of Medicine·L B Ware, M A Matthay
May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·G M BoylanM C McElroy
Jun 1, 2001·The Journal of Laboratory and Clinical Medicine·E N AtochinaM F Beers
Jan 16, 2002·American Journal of Respiratory and Critical Care Medicine·James A FrankMichael A Matthay
Jun 18, 2002·Infection and Immunity·Mary C McElroyChristopher Haslett
Mar 26, 2003·Developmental Biology·Maria I RamirezMary C Williams
Oct 2, 2004·The European Respiratory Journal·M C McElroy, M Kasper
Apr 26, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Gareth R CleggMary C McElroy
Nov 1, 2005·Respiratory Research·R JounblatA Kadioglu
Jan 20, 2006·Briefings in Functional Genomics & Proteomics·Aras Kadioglu, Peter W Andrew
Mar 24, 2007·Respiratory Medicine·Francesco BlasiPaolo Tarsia
Mar 30, 2007·The American Journal of Pathology·Akiko KunitaNaoya Fujita
Jul 10, 2007·The Journal of Biological Chemistry·Katsue Suzuki-InoueYukio Ozaki
Aug 30, 2008·American Journal of Respiratory Cell and Molecular Biology·Fernando A BozzaGuy A Zimmerman
Jan 17, 2009·The International Journal of Biochemistry & Cell Biology·Ester Martín-VillarJaime Renart
Mar 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ann M KerriganGordon D Brown
Feb 4, 2010·The Journal of Biological Chemistry·Yongquan ShenGary S Goldberg
Citations
Aug 13, 2015·Intensive Care Medicine Experimental·William R HendersonAndrew W Sheel
Jun 5, 2014·Molecular and Cellular Biochemistry·Li LinChang-chong Li
Jan 19, 2016·Toxicology and Applied Pharmacology·Feng WuMax Costa
Jun 18, 2016·European Journal of Immunology·Barbara B MaierSylvia Knapp
Oct 27, 2016·Respirology : Official Journal of the Asian Pacific Society of Respirology·Simone M C SpoorenbergUNKNOWN Ovidius Study Group
Oct 4, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andrew J ParisG Scott Worthen
Jan 13, 2018·PloS One·Simone M C SpoorenbergUNKNOWN Ovidius study group
Sep 30, 2020·Nature Cell Biology·Andrew J ParisG Scott Worthen
May 28, 2021·Frontiers in Immunology·Anna KosyrevaTimur Fatkhudinov