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Differential analysis of gene regulation at transcript resolution with RNA-seq

Nature Biotechnology

Dec 12, 2012

Cole TrapnellLior Pachter

Abstract

Differential analysis of gene and transcript expression using high-throughput RNA sequencing (RNA-seq) is complicated by several sources of measurement variability and poses numerous statistical challenges. We present Cuffdiff 2, an algorithm that estimates expression at transcript-leve...read more

Mentioned in this Paper

Specimen Type - Fibroblasts
HOXA1 protein, human
Lung
Sequence Determinations, RNA
Promoter
Nuclear mRNA Cis Splicing, via Spliceosome
HOXA1 gene
Lung Diseases
Homeodomain Proteins
RNA Splicing
62
3
3105
1
2
Paper Details
References
  • References50
  • Citations800
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  • References50
  • Citations800
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Differential analysis of gene regulation at transcript resolution with RNA-seq

Nature Biotechnology

Dec 12, 2012

Cole TrapnellLior Pachter

PMID: 23222703

DOI: 10.1038/nbt.2450

Abstract

Differential analysis of gene and transcript expression using high-throughput RNA sequencing (RNA-seq) is complicated by several sources of measurement variability and poses numerous statistical challenges. We present Cuffdiff 2, an algorithm that estimates expression at transcript-leve...read more

Mentioned in this Paper

Specimen Type - Fibroblasts
HOXA1 protein, human
Lung
Sequence Determinations, RNA
Promoter
Nuclear mRNA Cis Splicing, via Spliceosome
HOXA1 gene
Lung Diseases
Homeodomain Proteins
RNA Splicing
62
3
3105
1
2

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RNA Splicing (Keystone)

The majority of human genes that encode proteins undergo alternative pre-mRNA splicing and mutations that affect splicing are more prevalent than previously thought. Discover the latest research on RNA splicing and co-transcriptional splicing in this feed.

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Paper Details
References
  • References50
  • Citations800
12345
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