Differential and coordinated regulation of expression of norepinephrine transporter in catecholaminergic cells in culture

Brain Research
I MatsuokaK Kurihara

Abstract

The norepinephrine transporter (NET) terminates noradrenergic neurotransmission at synapse by high-affinity sodium-dependent reuptake into presynaptic terminals, and thus serves as a marker of differentiation of noradrenergic neurons. In the present study, we studied the regulatory mechanism of the expression of NET-mRNA in cultured neurons from newborn rat superior cervical ganglia (SCG) and in clonal rat pheochromocytoma cells (PC12) SCG neurons in culture expressed a high level of NET-mRNA, which was further increased 2.5-5 fold from day 1 to day 13. Treatment of SCG neurons with the cholinergic differentiation factor (CDF)/leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF), neurokines known to induce the switch from adrenergic to cholinergic phenotype in SCG neurons, led to the suppression of the level of NET-mRNA in a concentration dependent manner, concomitantly with the suppression of mRNA for tyrosine hydroxylase (TH), an adrenergic marker enzyme in cultured SCG neurons. On the other hand, retinoic acid, a compound which is also known to increase the expression of choline acetyltransferase, a cholinergic marker enzyme, and suppress the expression of TH in the cultured SCG neurons and PCI2 cells, rat...Continue Reading

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Citations

Jul 16, 2003·Journal of Neurochemistry·Wei LiBeth A Habecker
Feb 20, 2020·Neuroscience and Biobehavioral Reviews·Pu HuJiang-Ning Zhou
May 11, 2005·Progress in Neurobiology·Michelle A Lane, Sarah J Bailey
Aug 21, 2007·Progress in Neuro-psychopharmacology & Biological Psychiatry·J Douglas Bremner, Peter McCaffery

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