Differential and mutually exclusive expression of CD95 and CD95 ligand in epithelia of normal pancreas and chronic pancreatitis

Laboratory Investigation; a Journal of Technical Methods and Pathology
C HaselP Möller

Abstract

Acinar regression in chronic pancreatitis may be due to immune attack in parenchymal areas neoexpressing HLA-DR molecules. CD4+Th1 cytotoxic T cells induce apoptosis of their targets via oligomerizing CD95 (APO-1/Fas) death receptors on target cells by their CD95 ligand (CD95L). We determined the expression of CD95 and CD95L in epithelia of normal and chronically inflamed pancreatic tissues. We applied RT-PCR and Western blotting for CD95L expression profiles, serial frozen section immunohistochemistry to detect CD95, CD95L, and HLA-DR molecules, CD3, CD4, CD11c, and S-100 protein (S100p). Normal pancreases and chronic pancreatitis contain CD95L message and protein. Immunohistochemistry revealed a mutually exclusive expression of CD95 and CD95L. Physiologically, acini were CD95-/CD95L+, ducts were CD95-/CD95L-, and islets were CD95-/CD95L+. In areas of lymphohistiocytic infiltration, mainly consisting of CD3+CD4+ T cells and CD11c+, CD4+/-, S100p+ interstitial dendritic cells, and in areas of initial fibrosis, acini and ducts were HLA-DR+, acini CD95+/CD95L-, and ducts CD95+/CD95L-. Islet cells were CD95-/CD95L+ in both conditions. IFNgamma levels in protein lysates, as measured by an immunoassay, were significantly higher in c...Continue Reading

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Citations

Jun 17, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Cornelia HaselPeter Möller
Aug 16, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hanne Klonowski-StumpeDieter Häussinger
Jul 9, 2002·Clinical and Experimental Immunology·W-M QuM Nose
Aug 30, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Cornelia HaselPeter Möller
Jan 25, 2007·International Journal of Experimental Pathology·Shigeru TakahashiMinoru Wakita
Oct 31, 2006·World Journal of Gastroenterology : WJG·Alexander SchneiderPeter Feick
Mar 25, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Umesh K Bhanot, Peter Möller

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis