Differential and opposing regulation of PAI-1 promoter activity by estrogen receptor alpha and estrogen receptor beta in endothelial cells

Circulation Research
Layton Harris SmithDouglas E Vaughan

Abstract

To investigate the molecular mechanisms involved in the estrogen-dependent control of plasminogen activator inhibitor-1 (PAI-1) gene expression in vascular cells, we compared the transactivation properties of estrogen receptors (ERalpha and ERbeta) in regulating the activity of a human PAI-1 promoter reporter construct in transfected bovine aortic endothelial cells (BAECs). ERalpha increased PAI-1 promoter activity in BAECs by an estrogen-dependent mechanism, whereas ERbeta suppressed PAI-1 promoter activity by an estrogen-independent mechanism. The suppressive activity of ERbeta was dominant over the inductive activity of ERalpha. Mutation of a putative estrogen response element (ERE) located at position -427 in the proximal promoter abolished the ERalpha action without influencing the suppressive effects of ERbeta. Mutation of either AP1-like site did not eliminate the ERalpha or ERbeta actions at the PAI-1 promoter, suggesting that other promoter elements are involved in these responses. These mutations significantly reduced the -3.4kbp PAI-1 promoter response to serum. We concluded that ERalpha and ERbeta exert differential effects on the PAI-1 promoter activity in transfected BAECs. ERalpha activated the PAI-1 promoter thr...Continue Reading

References

Mar 1, 1970·Journal of Molecular Biology·S B Needleman, C D Wunsch
Sep 25, 1995·Biochemical and Biophysical Research Communications·T HayashiA Iguchi
Aug 15, 1996·The New England Journal of Medicine·F GrodsteinC H Hennekens
Jun 11, 1999·The New England Journal of Medicine·M E Mendelsohn, R H Karas
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·R H KarasM E Mendelsohn
Jun 15, 2000·The New England Journal of Medicine·H P Kohler, P J Grant
Oct 24, 2000·Oncogene·K PetterssonJ A Gustafsson
Jan 6, 2001·VASA. Zeitschrift für Gefässkrankheiten·M JoswigP P Nawroth
Feb 13, 2001·The Journal of Steroid Biochemistry and Molecular Biology·B S KatzenellenbogenJ A Katzenellenbogen
Feb 13, 2001·The Journal of Steroid Biochemistry and Molecular Biology·P J KushnerP Webb
Dec 12, 2001·The Journal of Endocrinology·M LiangB O Nilsson
May 3, 2002·The Journal of Biological Chemistry·Meng-Min LiuPeter J Kushner
Jul 19, 2002·JAMA : the Journal of the American Medical Association·Jacques E RossouwUNKNOWN Writing Group for the Women's Health Initiative Investigators
May 10, 2003·Journal of Molecular and Cellular Cardiology·John A SchoenhardDouglas E Vaughan

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Citations

Mar 6, 2012·Molecular Human Reproduction·Santhosh GopalTommaso Simoncini
Jun 7, 2006·Critical Care Medicine·Patricia A CristofaroHeather A Harris
Feb 7, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Paul D DiMustoGilbert R Upchurch
Aug 30, 2008·The Journal of Endocrinology·Seiji TsutsumiMichael E Mendelsohn
Oct 22, 2008·The Journal of Endocrinology·Vincent RicchiutiGail K Adler
Nov 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jun Ming WangRoberta Diaz Brinton
Nov 26, 2015·International Journal of Molecular Sciences·Silvia Hoirisch-Clapauch, Antonio E Nardi
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·D E Vaughan
Oct 21, 2010·Clinical Science·Amparo C VillablancaCarole Banka
Oct 12, 2014·Human Reproduction Update·D K HapangamaJ N Bulmer
Feb 10, 2017·Headache·Gretchen E Tietjen, Stuart A Collins
Feb 18, 2005·Journal of Lipid Research·Layton Harris SmithDouglas E Vaughan
Aug 19, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Chiara BolegoAndrea Cignarella
Aug 7, 2004·Circulation Research·Nageswara MadamanchiMarschall S Runge
Oct 23, 2010·Expert Review of Molecular Diagnostics·Karin MengeleManfred Schmitt
Dec 21, 2019·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Adeyanju Oluwaseun AremuOlatunji Lawrence Aderemi
Jan 4, 2021·Breast Cancer Research and Treatment·Elena E TzekakiAngelos Papaspyropoulos
Jul 30, 2009·Physiology & Behavior·Esther L SabbanLidia I Serova

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