Differential assembly of cytoskeletal and sarcomeric actins in developing skeletal muscle cells in vitro

Zoological Science
K HayakawaTakashi Obinata

Abstract

Monoclonal antibodies (McAb) to actin were prepared to analyze the assembly of actin isoforms in developing muscle cells in vitro. One of the antibodies (SkA-06) was specific for alpha-sarcomeric actin isoforms in skeletal and cardiac muscles, while the others recognized cytoskeletal (beta, gamma) actin isoforms in smooth muscle and non-muscle tissues as well as the sarcomeric (alpha) actins. Using SkA-06 and a polyclonal antibody (PcAb) specific for cytoskeletal actins, the subcellular localization of the actin isoforms was examined by immunocytochemical methods. While in developing young myotubes, cytoskeletal and sarcomeric actins were co-localized in nascent myofibrils or stress-fiber-like structures, sarcomeric actins predominated in striated myofibrils in more developed myotubes. When FITC-labeled cytoskeletal and sarcomeric actins were introduced into young myotubes by a microinjection method, the latter became detectable in striated structures sooner than the former but they were finally incorporated into striated myofibrils. These results suggest that alpha-actin(s) as well as beta- and gamma-actins can be incorporated into myofibrils, but alpha-actin(s) is assembled preferentially into myofibrils in developing muscle ...Continue Reading

References

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Citations

Jan 19, 1999·Annual Review of Cell and Developmental Biology·R Littlefield, V M Fowler
Oct 30, 2004·Biological Procedures Online·Heidi RommelaereChristophe Ampe
Sep 8, 2007·Journal of Muscle Research and Cell Motility·Atsuko HosodaTakashi Obinata
Sep 20, 2017·Circulation Research·Wassim A BasheerRobin M Shaw
Mar 8, 2014·Zoological Science·Takashi ObinataNaruki Sato

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Methods Mentioned

BETA
gel filtration
electrophoresis
GAM
mechanical dissociation
ELISA
transfection

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Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.

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