Differential bacterial gene expression during experimental pneumococcal endophthalmitis

Ophthalmic Research
Justin A ThorntonMary E Marquart

Abstract

Streptococcus pneumoniae (pneumococcus) is a potential cause of bacterial endophthalmitis in humans that can result in ocular morbidity. We sought to identify pneumococcal genes that are differentially expressed during growth in the vitreous humor of the eye in an experimental endophthalmitis model. Microarray analysis was used to identify genes that were differentially expressed when pneumococci replicated in the vitreous of rabbit eyes as compared with bacteria grown in vitro in Todd Hewitt medium. Array results were verified by quantitative real-time PCR analysis of representative genes. Select genes potentially playing a role in virulence during endophthalmitis were deleted, and mutants were tested for reduced eye pathogenesis and altered adhesion to host cells. Array analysis identified 134 genes that were differentially expressed during endophthalmitis; 112 genes demonstrated increased expression during growth in the eye whereas 22 were downregulated. Real-time analysis verified increased expression of neuraminidase A (NanA; SP1693), neuraminidase B (NanB; SP1687) and serine protease (SP1954), and decreased expression of RlrA (SP0461) and choline transporter (SP1861). Mutation of NanA and NanB had no major effect on patho...Continue Reading

Citations

May 8, 2016·Progress in Retinal and Eye Research·Roger A AstleyMichelle C Callegan
May 17, 2019·MSphere·Phillip S CoburnMichelle C Callegan
Dec 13, 2018·Interdisciplinary Perspectives on Infectious Diseases·Angela H Benton, Mary E Marquart

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