Differential Behavior Between Isolated and Aggregated Rabbit Auricular Chondrocytes on Plastic Surfaces

Journal of Biomedicine & Biotechnology
Vega Villar-SuárezJ M Villar-Lacilla

Abstract

A knowledge of the behavior of chondrocytes in culture is relevant for tissue engineering. Chondrocytes dedifferentiate to a fibroblast-like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured in suspension. In this report a description is given of how when chondrocyte aggregates formed in suspension are next seeded on plastic, most of them attach as round or polygonal cells. This morphological differentiation, with synthesis of type II collagen, is stable for long culture periods. This simple method can be of use as a model for studies of chondrocyte behavior on plastic. The results indicate that in addition to culture conditions, such as cell isolation method or cell density, chondrocyte behavior on plastic depends on the presence of aggregates.

Citations

Jun 19, 2009·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Amir AtashiMasoud Soleimani
Dec 3, 2010·Biotechnology Letters·Tae-Jin LeeByung-Soo Kim
Sep 10, 2008·IEEE Transactions on Nanobioscience·Suncica Canić, Doreen Rosenstrauch
Sep 29, 2011·Analytical Chemistry·Ali YilmazJerzy W Jaroszewski
Jan 19, 2008·Journal of Cognitive Neuroscience·Tamara C Cristescu, Anna Christina Nobre
Feb 20, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Richard L SurmaitisJoseph B Schlenoff

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