Differential behaviour of epicardial adipose tissue-secretomes with high and low orosomucoid levels from patients with cardiovascular disease in H9C2 cells

Molecular and Cellular Endocrinology
Ricardo LageSonia Eiras

Abstract

Epicardial adipose tissue releases orosomucoid (ORM), an acute phase protein with multiple modulatory and protective properties. We aimed to identify the effect of EAT-supernatants according to their ORM levels on H9C2 cells. H9C2 were cultured with EAT-secretomes or ORM protein itself on a Real-Time Cell Analyser. Secretome proteins identification was performed by LC-mass spectrometry according to their ORM levels. Two of them were validated by ELISA in EAT-supernatants from 42 patients. ORM effect on H9C2 and neonatal rat cardiomyocytes apoptosis under hypoxia with or without fatty acid treatment was determined by Annexin-V flow cytometry measurement. Caspase-3 expression levels were determined by western blot in H9C2. Our results showed a differential effect of EAT-secretomes according their ORM levels. Although additional secreted proteins can contribute to their beneficial effects, ORM reduced hypoxia-induced apoptosis through caspase-3 inhibition. Our data showed the cardioprotective role of ORM and suggest that its quantification on EAT secretomes might help us to find new secreted factors with a cardioprotective role.

References

Aug 3, 1999·The Journal of Clinical Investigation·S FrantzR A Kelly
Nov 4, 2000·Biochimica Et Biophysica Acta·T FournierD Porquet
Oct 29, 2003·Circulation·Tomasz MazurekYi Shi
Aug 11, 2005·The Journal of Clinical Endocrinology and Metabolism·Gianluca Iacobellis, Frida Leonetti
Apr 21, 2007·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·S W Rabkin
Jun 9, 2007·Journal of Endocrinological Investigation·M K HeliövaaraP Ebeling
Jun 29, 2007·Bioinformation·Mehdi PiroozniaYouping Deng
Jan 9, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·J N FainA K Madan
Aug 23, 2008·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·S ErogluH Muderrisoglu
May 13, 2010·Biomedical Research·Takashi MuraguchiShunichiro Kubota
Jul 14, 2010·Journal of Cardiovascular Magnetic Resonance : Official Journal of the Society for Cardiovascular Magnetic Resonance·Christina DoeschTheano Papavassiliu
Sep 3, 2010·Molecular & Cellular Proteomics : MCP·Elena Bonzon-KulichenkoJesús Vázquez
May 5, 2011·Journal of Visualized Experiments : JoVE·Aja M RiegerDaniel R Barreda
May 14, 2011·The American Journal of Cardiology·Tuba KhawajaP Christian Schulze
May 18, 2011·Cardiovascular Research·Carolina Gálvez-MontónAntoni Bayes-Genis
Aug 20, 2011·Trends in Endocrinology and Metabolism : TEM·Gianluca Iacobellis, Antonio C Bianco
Oct 13, 2011·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Fu-Jun ShangLian-You Zhao
Oct 16, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Shu Q LiuYu H Wu
Mar 7, 2013·The Journal of Biological Chemistry·Daniel Perez-HernandezMaría Yáñez-Mó
Mar 20, 2013·Current Heart Failure Reports·Konstantinos Drosatos, P Christian Schulze
Apr 29, 2014·Journal of the American College of Cardiology·Yoshiyuki KataokaNoriyuki Ouchi
Oct 24, 2014·Frontiers in Immunology·Ardiyanto Liaunardy-Jopeace, Nicholas J Gay

❮ Previous
Next ❯

Citations

Dec 15, 2015·Apoptosis : an International Journal on Programmed Cell Death·Zhuqing JiaChunyan Zhou
Aug 5, 2016·Cell Proliferation·Minjia DaiWeidong Tian
Feb 14, 2017·The Journal of Physiology·Alexios S Antonopoulos, Charalambos Antoniades
Jan 13, 2018·Journal of Asian Natural Products Research·Mao-Shan YinYan-Ling Mu
Dec 31, 2016·Journal of Cardiovascular Translational Research·Sonia EirasJosé R González-Juanatey
Apr 6, 2019·Current Protein & Peptide Science·Fabrizio Ceciliani, Cristina Lecchi
Nov 30, 2019·Clinical Science·Sarah R AnthonyMichael Tranter
Jan 4, 2021·Stem Cell Reviews and Reports·Yan LinYao Xie

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.

Atrial Filbrillation

Atrial fibrillation refers to the abnormal heart rhythm characterized by rapid and irregular beating of the atria. Here is the latest research.

Cardiovascular Inflammation

Inflammation plays a significant role in the development of cardiovascular diseases, an understanding of these endogenous processes is critical for evaluating the risks and potential treatment strategies. Discover the latest research on cardiovascular inflammation here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Related Papers

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Li ZhangHongli Shan
Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
Fang ZhaoShi-xi Xiong
© 2021 Meta ULC. All rights reserved