Differential binding and regulation of platelet-derived growth factor A and B chain isoforms by alpha 2-macroglobulin.

The Journal of Biological Chemistry
J C Bonner, A R Osornio-Vargas

Abstract

Alpha 2-Macroglobulin (alpha 2M) is a multifunctional secreted glycoprotein that serves as a ubiquitous proteinase inhibitor and as a binding protein for platelet-derived growth factor (PDGF) BB and homologues of PDGF-BB secreted in culture by macrophages. The interaction of alpha 2M with PDGF-A chain molecules has not been addressed. This is a potentially important issue because fibroblasts and smooth muscle cells produce PDGF-AA, whereas macrophages produce mainly PDGF-BB. Recombinant human 125I-PDGF-B chain molecules (AB and BB) bound to plasma-derived, native human, or bovine alpha 2M and trypsin-activated alpha 2M on Superose 6 fast protein liquid chromatography gel filtration and on nondenaturing polyacrylamide gel electrophoresis, whereas 125I-PDGF-AA did not. Similar results were obtained with 125I-PDGF isoforms binding to immobilized bovine alpha 2M and alpha 2M-methylamine. The same differential pattern of unlabeled PDGF isoforms binding to alpha 2M was observed by Western blotting of PDGF. Human lung fibroblasts secreted alpha 2M as measured by Western blotting, and fibroblast-derived alpha 2M possessed the same differential binding pattern for PDGF isoforms as did plasma-derived alpha 2M. The specific binding of PDG...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E W RainesE H Sage
Feb 1, 1992·Archives of Biochemistry and Biophysics·D J WebbS L Gonias
Dec 1, 1990·American Journal of Respiratory Cell and Molecular Biology·A R Osornio-VargasA R Brody
Oct 1, 1990·The Journal of Clinical Investigation·H N AntoniadesM Selman
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L Claesson-WelshC H Heldin
Jun 10, 1988·Science·C E HartD F Bowen-Pope
Nov 1, 1982·Journal of Cellular Physiology·G R GrotendorstG R Martin
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J S HuangT F Deuel
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E W RainesR Ross
Feb 1, 1982·The Journal of Cell Biology·H SeppäG R Martin
Jul 7, 1995·The Journal of Biological Chemistry·J C Bonner, A R Osornio-Vargas

❮ Previous
Next ❯

Citations

Mar 14, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·T R GarberD J Webb
May 17, 2008·Genes & Development·Johanna AndraeChrister Betsholtz
Jun 11, 2014·The Journal of Oral Implantology·Prasun ShahJames Rutkowski
May 27, 2006·Endothelium : Journal of Endothelial Cell Research·Marco Rusnati, Marco Presta
Jun 23, 2004·Cytokine & Growth Factor Reviews·James C Bonner
May 1, 2002·Nature Biotechnology·Simon FredrikssonUlf Landegren
Oct 3, 1999·Physiological Reviews·C H Heldin, B Westermark
Jul 7, 1995·The Journal of Biological Chemistry·J C Bonner, A R Osornio-Vargas

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.