Differential binding of the NFE3 and CP1/NFY transcription factors to the human gamma- and epsilon-globin CCAAT boxes.

The Journal of Biological Chemistry
A RonchiC Santoro

Abstract

Naturally occurring nondeletional mutations affecting the distal CCAAT box of the human gamma-globin gene promoter result in hereditary persistence of fetal hemoglobin in adult life. Although the distal CCAAT box is the target of several factors, including CP1/NFY, CDP, GATA-1 and NFE3, only NFE3 binding activity is consistently sensitive to well characterized mutations in this region such as G-117-->A, C-114-->T, and delta 13 hereditary persistence of fetal hemoglobin. We extensively characterized the binding specificities of NFE3 and demonstrated that NFE3 has unique properties with respect to other CCAAT box-binding proteins. Affinity-purified NFE3 from erythroid K562 cells binds the distal but not the proximal human gamma-globin CCAAT box, the single CCAAT box of the human epsilon-globin promoter, and the proximal CCAAT box of the evolutionarily related Galago crassicaudatus gamma-globin gene. Within the epsilon-globin CCAAT box, NFE3 represents the major and almost exclusive binding activity. Disruption of such a binding site essentially inactivates the epsilon-globin promoter, suggesting that NFE3 plays an important role in the embryonic expression of this gene.

References

Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T Ikuta, Y W Kan
May 25, 1989·Nucleic Acids Research·F CatalaF Grosveld
Sep 25, 1987·Nucleic Acids Research·R A Hooft van HuijsduijnenD Mathis
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J L ManleyM L Gefter
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D L GumucioM Goodman

❮ Previous
Next ❯

Citations

Nov 1, 1996·The International Journal of Biochemistry & Cell Biology·S M Jane, J M Cunningham
Jul 25, 2000·Nucleic Acids Research·N P DaviesV Murray
Dec 22, 2006·PloS One·Alon GorenHoward Cedar
Sep 15, 2000·Journal of Molecular Biology·G CarettiR Mantovani
Apr 14, 2007·The EMBO Journal·Osamu TanabeJames Douglas Engel
Oct 22, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Xin-Jun JiChih-chuan Liang
Jun 27, 1998·The Journal of Biological Chemistry·C LiberatiR Mantovani
Feb 29, 2020·Haematologica·Cristina FugazzaAntonella Ellena Ronchi
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Gloria BarbaraniAntonella E Ronchi

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.