Differential biological effects of 1,25-dihydroxyVitamin D3 on melanoma cell lines in vitro

The Journal of Steroid Biochemistry and Molecular Biology
Markus SeifertJörg Reichrath

Abstract

1,25-DihydroxyVitamin D(3) and analogs have been shown to inhibit proliferation and to induce differentiation in different cell types, including human melanocytes. However, various tumor cell lines that fail to respond to the antiproliferative effects of Vitamin D analogs have also been reported. Using real-time PCR (LightCycler), we have compared mRNA expression of Vitamin D receptor (VDR), Vitamin D-25-hydroxylase (25-OHase), 25-hydroxyVitamin D-1alpha-hydroxylase (1alpha-OHase), and 1,25-dihydroxyVitamin D-24-hydroxylase (24-OHase) in a melanoma cell line that responds to antiproliferative effects of Vitamin D (MeWo) with a non-responsive melanoma cell line (SkMel5). Additionally, modulation of cell proliferation by calpain inhibitors, as well as regulation of mRNA expression of VDR, 1alpha-OHase, and 24-OHase genes by Vitamin D analogs were assessed in melanoma cell lines in vitro using a WST-1 based colorimetric assay and real-time PCR, respectively. RNA for VDR, 25-OHase, 1alpha-OHase, and 24-OHase was detected in melanoma cell lines. In contrast to SkMel5 cells, treatment of MeWo cells with calcitriol resulted in a dose-dependent increase in mRNA for VDR and 24-OHase as well as in a suppression of cell proliferation (up ...Continue Reading

References

May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A R BakerB W O'Malley
Feb 7, 1998·The Biochemical Journal·H SorimachiK Suzuki
Apr 21, 1999·International Journal of Cancer. Journal International Du Cancer·C DanielssonC Carlberg
Jul 5, 2001·Frontiers in Bioscience : a Journal and Virtual Library·C M HansenC Carlberg
Jun 20, 2002·The Journal of Biological Chemistry·Ida Stenfeldt MathiasenMarja Jäättelä

❮ Previous
Next ❯

Citations

Jun 29, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jean Y TangMarcia L Stefanick
Dec 8, 2009·Endocrinology·Shuanhu ZhouJulie Glowacki
Jan 14, 2016·International Journal of Molecular Sciences·Anna PiotrowskaMichał A Żmijewski
Oct 16, 2012·Journal of the American Academy of Dermatology·Jean Y TangMaryam M Asgari
Oct 16, 2012·Journal of the American Academy of Dermatology·Jean Y TangMaryam M Asgari
May 16, 2012·Journal of the American Academy of Dermatology·Katherine A WardMaria K Hordinsky
Feb 16, 2010·The Journal of Steroid Biochemistry and Molecular Biology·S EssaJ Reichrath
Mar 9, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Sandra ReichrathJörg Reichrath
Feb 6, 2009·The Journal of Investigative Dermatology·Maryam M AsgariEmily White
Sep 23, 2006·The Journal of Investigative Dermatology·Chunying LiQingyi Wei
Nov 26, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Christina KrämerJörg Reichrath
Jan 17, 2007·The British Journal of Dermatology·C SantonocitoE Capoluongo
Jan 10, 2008·International Journal of Cancer. Journal International Du Cancer·Chunying LiQingyi Wei
Sep 3, 2010·Dermato-endocrinology·Jörg Reichrath, Bernd Nürnberg
Mar 5, 2011·Molecular Oncology·Sinead Field, Julia A Newton-Bishop
Aug 25, 2016·PloS One·Sang Min ParkEunyoung Cho
Jan 30, 2015·Einstein·Renato Santos de Oliveira FilhoCarlos Eduardo dos Santos Ferreira
Jul 28, 2015·Cancers·Irene RussoMauro Alaibac
Oct 29, 2017·Photodermatology, Photoimmunology & Photomedicine·Hala M El-HanbuliRania H Mahmoud
Jan 30, 2013·Molecular Carcinogenesis·Natalie NemazannikovaCrispin R Dass
May 14, 2008·Experimental Dermatology·Christos C ZouboulisAnja Thielitz
Nov 23, 2006·Photochemistry and Photobiology·Robert M Sayre, John C Dowdy
Sep 29, 2010·Experimental Dermatology·Joel Pinczewski, Andrzej Slominski
Mar 21, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yuan GaoJulie Glowacki
May 19, 2012·Expert Review of Anticancer Therapy·Paulina SzyszkaAndrzej T Slominski
Feb 22, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Andrzej T SlominskiCraig A Elmets
Aug 2, 2018·International Journal of Molecular Sciences·Ewa PodgorskaKrystyna Urbanska
Sep 12, 2018·International Journal of Molecular Sciences·Tomasz WasiewiczMichal A Zmijewski
Feb 1, 2015·Melanoma Management·Candy WyattRobyn M Lucas
Apr 4, 2015·Cancer Prevention Research·Clara Curiel-LewandrowskiH-H Sherry Chow
Nov 20, 2020·Scientific Reports·Jo S StenehjemTrude E Robsahm
Jul 25, 2014·Archives of Biochemistry and Biophysics·Adi Nosrati, Maria L Wei

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2022 Meta ULC. All rights reserved