Differential blood transcriptome modules predict response to corticosteroid therapy in alcoholic hepatitis.

JHEP Reports : Innovation in Hepatology
Shvetank SharmaShiv K Sarin

Abstract

In patients with severe alcoholic hepatitis (SAH), little is known about the profile of peripheral blood mononuclear cells (PBMCs) at baseline and during corticosteroid therapy, among those who can be treated successfully with steroids (steroid-responders [R] and those who cannot (steroid-non-responders [NR]); 2 groups with different outcomes. We performed RNA-seq analysis in PBMCs from 32 patients with definite SAH, at baseline and after 7 days of corticosteroids. The data were sorted into R and NR (n = 16, each group) using the Lille model and 346 blood transcription modules (BTMs) were identified. BTMs are predefined modules of highly co-expressed PBMC genes, which can determine specific immune cell types and cellular functions. The activity of each BTM was taken as the mean value of its member genes. At baseline, 345 BTMs had higher activity (i.e. were upregulated) in NR relative to R. The 100 most upregulated BTMs in NR, included several modules related to lymphoid lineage (T, B, and natural killer [NK] cells), modules for cell division and mitochondrial respiratory electron transport chain (ETC, relating to energy production), but only a few modules of myeloid cells. Correlation studies of BTM activities found features of...Continue Reading

References

Jan 1, 1986·Journal of Hepatology·K T Nouri-AriaR Williams
Aug 31, 1974·Lancet·I M BernsteinR G Strickland
May 24, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alexandre LouvetPhilippe Mathurin
Mar 4, 2010·Genome Biology·Mark D Robinson, Alicia Oshlack
Oct 12, 2013·Science·Erika L PearceRussell G Jones
Apr 23, 2015·The New England Journal of Medicine·Mark R ThurszUNKNOWN STOPAH Trial
Dec 24, 2015·The Journal of Experimental Medicine·Luke A J O'Neill, Edward J Pearce
Mar 6, 2017·Gastroenterology·Ashwani K SingalPatrick S Kamath
May 16, 2017·Cell·Shuzhao LiBali Pulendran
Jun 9, 2018·Hepatology Communications·Jaswinder Singh MarasShiv Kumar Sarin
Jan 12, 2019·Science·Andrew WangRuslan Medzhitov
Mar 12, 2019·Cell·Kirthana GaneshanAjay Chawla
Apr 20, 2019·Cell·Jason G Cyster, Christopher D C Allen
May 8, 2019·Nature Biotechnology·Aaron M NewmanAsh A Alizadeh

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Citations

May 11, 2021·JHEP Reports : Innovation in Hepatology·Sven M A Francque

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Software Mentioned

FlowJo
RStudio
ABIS
Bowtie21
R
R package ‘ linkcomm ’
Morpheus
DESeq2
Reactome
R Foundation for Statistical Computing

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