Differential Ca2+ signaling induced by activation of the epidermal growth factor and nerve growth factor receptors.

The Journal of Biological Chemistry
I TinhoferH H Grunicke

Abstract

Stimulation by epidermal growth factor (EGF) of NIH3T3 cells overexpressing the EGF receptor (EGFR) results in a release of Ca2+ from internal stores. Ca2+ release is followed by an influx of extracellular calcium which can be recorded by the influx of the calcium surrogate Mn2+. Both Ca2+ release and Mn2+/Ca2+ influx are inhibited by expression of the dominant negative Asn17-Ras mutant and abrogated by microinjected neutralizing anti-Ras antibody Y13-259, whereas microinjection of the anti-Ras antibody Y13-238 which does not interact with the effector binding domain of Ras is without any effect on the EGF-induced Ca2+ transient. Neither Asn17-Ha-Ras nor the Y13-259 antibody interferes with the thapsigargin-induced Mn2+/Ca2+ influx. The nerve growth factor receptor (Trk)-mediated Ca2+ transient was found to be unaffected by the dominant negative Ras mutant or microinjected neutralizing anti-Ras antibodies. Substitution of the phospholipase Cgamma1 (PLCgamma1) binding site of the EGFR by the PLCgamma binding domain of Trk renders the EGFR-induced Ca2+ influx insensitive to the expression of Asn17-Ha-Ras, whereas the Ca2+ signal induced by Trk carrying the PLC binding site of EGFR is Ras-dependent and abrogated by the dominant ne...Continue Reading

References

Aug 1, 1995·Cell Calcium·K MalyH H Grunicke
Jun 16, 1995·Cell·M P PeppelenboschJ L Bos
Jan 27, 1995·Cell·D E Clapham
Feb 23, 1995·Nature·R HeimR Y Tsien
Jan 1, 1994·Annual Review of Cell Biology·P van der GeerR A Lindberg
Oct 1, 1993·Endocrine Reviews·J W Putney, G S Bird
Jan 28, 1993·Nature·M J Berridge
Apr 5, 1996·The Journal of Biological Chemistry·M P PeppelenboschJ L Bos
Apr 1, 1996·Current Opinion in Cell Biology·C J Marshall

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Feb 19, 1998·Archives of Biochemistry and Biophysics·R GoldmanU Zor
Jan 27, 1999·Biochimica Et Biophysica Acta·D J Gawler

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