Differential calcium independent regulation of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases by interleukin-1beta and transforming growth factor-beta in Peyronie's plaque fibroblasts

The Journal of Urology
Marcello Del CarloLaurence A Levine

Abstract

Peyronie's disease is a fibrotic disorder of the tunica albuginea characterized by the localized formation of an inelastic plaque. We characterized matrix metalloproteinases and TIMPs (tissue inhibitors of matrix metalloproteinase) in Peyronie's disease tissue. Matrix metalloproteinases and TIMPs were investigated in Peyronie's disease plaque tunica removed from patients with stable Peyronie's disease. Immunological methods were used to characterize the matrix metalloproteinases and TIMPs produced by cell cultures stimulated with transforming growth factor-beta or interleukin-1beta (PreproTech, Rocky Hill, New Jersey). Enzyme activity was quantified with a fluorescent substrate and correlated with mRNA levels using real-time polymerase chain reaction. Interleukin-1beta significantly induced (p <0.01) matrix metalloproteinase-1, 3, 10 and 13 protein production, endogenous matrix metalloproteinase-13 activity (12-fold) and matrix metalloproteinase-13 mRNA expression (11.2-fold) through a Ca(2+) independent mechanism in cultured fibroblasts. Transforming growth factor-beta stimulation failed to induce any detectable matrix metalloproteinase protein production or activity and conditioned culture medium even had the capacity to inhi...Continue Reading

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Sep 16, 2011·International Journal of Impotence Research·S M Larsen, L A Levine
Feb 24, 2016·Urology·Benjamin A Sherer, Laurence A Levine
Oct 28, 2016·Sexual Medicine Reviews·Kevin K Yang, Nelson Bennett
Jul 24, 2012·The Journal of Urology·Laurence A Levine, Raymond A Costabile
Nov 6, 2009·The Journal of Sexual Medicine·Ling X De YoungGerald B Brock
Jun 30, 2010·The Journal of Sexual Medicine·Markos KaravitakisGregorios Doumanis
Jan 14, 2009·The Journal of Sexual Medicine·Nestor F Gonzalez-Cadavid, Jacob Rajfer
May 26, 2010·The Journal of Sexual Medicine·David RalphLaurence Levine
May 1, 2015·Expert Opinion on Pharmacotherapy·Benjamin A ShererLaurence A Levine
Apr 16, 2011·Expert Opinion on Therapeutic Targets·Ibrahim A Abdel-Hamid, Tarek Anis
Jan 12, 2005·Arthritis Research & Therapy·Wan-Uk KimChul-Soo Cho
Jul 11, 2014·International Braz J Urol : Official Journal of the Brazilian Society of Urology·M SalehipourM R Farsiani
Jun 2, 2016·Therapeutic Advances in Urology·Elizabeth J TraoreWayne J G Hellstrom
Jul 18, 2018·Expert Review of Medical Devices·Shaan A Setia, Laurence A Levine
Mar 10, 2010·Nature Reviews. Urology·Nestor F Gonzalez-Cadavid, Jacob Rajfer
Mar 1, 2019·Investigative Ophthalmology & Visual Science·David TroiloLyndon Jones
Oct 17, 2019·International Urology and Nephrology·Ahmed Mohamed Hassanin, Ahmed Zain Abdel-Hamid
Apr 8, 2020·International Journal of Urology : Official Journal of the Japanese Urological Association·Matthew J ZiegelmannLaurence A Levine
Jul 11, 2020·The World Journal of Men's Health·Fabrizio Di MaidaAndrea Cocci
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Sep 1, 2013·Arab Journal of Urology·Laurence A Levine
Nov 7, 2018·Nature Reviews. Urology·Peter Tsambarlis, Laurence A Levine
Jan 22, 2021·Urology·Quinn C RainerThomas A Masterson
Mar 16, 2021·Expert Review of Clinical Pharmacology·Eric V LiLaurence A Levine
Jun 8, 2021·Frontiers in Pharmacology·Fuxun ZhangJiuhong Yuan

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