PMID: 9555064May 23, 1998Paper

Differential catalytic properties in metabolism of endogenous and exogenous substrates among CYP3A enzymes expressed in COS-7 cells

Biochimica Et Biophysica Acta
S OhmoriM Kitada

Abstract

The catalytic properties of CYP3A7 in the metabolism of endogenous and exogenous substrates were compared with those of CYP3A4 and CYP3A5 using COS-7 expressing enzymes. The highest activities of dehydroepiandrosterone (DHEA) and dehydroepiandrosterone 3-sulfate (DHEA-S) 16alpha-hydroxylase were observed in COS-7 cells expressing CYP3A7. In contrast, the activity of testosterone 6beta-hydroxylase of CYP3A7 expressed in COS-7 cells was much less than that of CYP3A4 expressed in COS-7 cells. The rate of carbamazepine 10, 11-epoxidation was the greatest in COS-7 cells expressing CYP3A4, followed by CYP3A5 and CYP3A7. On the other hand, the formation of reductive metabolite of zonisamide was the highest in COS-7 cells expressing CYP3A4, followed by CYP3A7 and CYP3A5. Furthermore, the addition of triazolam resulted in a decrease in 6beta-hydroxylation catalyzed by CYP3A7, but not by CYP3A4, whereas the pretreatment of microsomes with triacetyloleandomycin (TAO) resulted in a decrease in the reaction catalyzed by CYP3A4, but not by CYP3A7. Together with these results, it was suggested that CYP3A7 exerts differential catalytic properties not only in metabolism of endogenous substrates but also in drug metabolism compared to CYP3A4 and...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·B J Clark, M R Waterman
Oct 1, 1991·Archives of Biochemistry and Biophysics·D J WaxmanK Korzekwa
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T Shimada, F P Guengerich
Jan 1, 1989·Archives of Biochemistry and Biophysics·S A Wrighton, M Vandenbranden
Jan 1, 1989·Annual Review of Pharmacology and Toxicology·F P Guengerich
Nov 1, 1989·Archives of Biochemistry and Biophysics·J D SchuetzP S Guzelian
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D T MolowaP S Guzelian
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P H BeauneF P Guengerich
Aug 15, 1985·Archives of Biochemistry and Biophysics·M KitadaY Kanakubo
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe

❮ Previous
Next ❯

Citations

Apr 8, 2003·European Journal of Pharmacology·Solveigh KrusekopfUllrich Kleeberg
Feb 3, 2004·Environmental Health Perspectives·Gary GinsbergBabasaheb Sonawane
Jan 9, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sonia R Miranda, Sharon A Meyer
Nov 19, 2013·The Prostate·Declan DohertyPaul Daniel Thompson
Sep 18, 2007·Expert Review of Neurotherapeutics·Hideto Miwa
Aug 23, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Shosaku KashiwadaSeth W Kullman
May 29, 2009·Expert Opinion on Drug Metabolism & Toxicology·Yan-Yan Zhang, Ling Yang
Jul 2, 2004·Therapeutic Drug Monitoring·Leszek Wojnowski
Jan 25, 2008·Pharmacogenetics and Genomics·Huan QiuLeszek Wojnowski
Jul 22, 2009·Clinical Neuropharmacology·Mohd Makmor-BakryMartin J Brodie
Jun 7, 2005·The Journal of Biological Chemistry·Cristina Rodriguez-AntonaMagnus Ingelman-Sundberg
Nov 30, 2005·The Pharmacogenomics Journal·E E ThompsonA Di Rienzo
Oct 8, 2017·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Sylvie E KandelJed N Lampe
Jan 24, 2002·The Journal of Pharmacology and Experimental Therapeutics·Ronald N Hines, D Gail McCarver
Apr 18, 2002·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·G G GibsonW El-Sankary
Mar 19, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Hiroyoshi NakamuraMitsukazu Kitada
Dec 21, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Nao TorimotoMitsukazu Kitada
May 4, 2010·Journal of Pediatric Endocrinology & Metabolism : JPEM·Hui WangJiang Yue
Apr 10, 2002·The Journal of Biological Chemistry·Oliver BurkLeszek Wojnowski
Sep 21, 2019·Biochemical and Biophysical Research Communications·Karl W SmithDiego F Cobice
Jan 8, 2021·Frontiers in Neuroscience·Chengqian LiAnmu Xie
Apr 19, 2021·Drug Metabolism and Pharmacokinetics·Yasushi Yamazoe, Masahiro Tohkin
Mar 10, 2000·Biochemical and Biophysical Research Communications·E InoueT Kamataki
Jun 3, 2021·International Journal of Molecular Sciences·Irina F Sevrioukova
Jul 14, 2021·The Journal of Clinical Endocrinology and Metabolism·Daniel SchmitzÅsa Johansson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.