Differential cell adhesion to vocal fold extracellular matrix constituents

Matrix Biology : Journal of the International Society for Matrix Biology
Tannin J FujaIngo R Titze

Abstract

The human vocal folds are a complex layering of cells and extracellular matrix. Vocal fold extracellular matrix uniquely contributes to the biomechanical viscoelasticity required for human phonation. We investigated the adhesion of vocal fold stellate cells, a novel cell type first cultured by our laboratory, and fibroblasts to eight vocal fold extracellular matrix components: elastin, decorin, fibronectin, hyaluronic acid, laminin and collagen types I, III and IV. Our data demonstrate that these cells adhere differentially to said substrates at 5 to 120 min. Cells were treated with hyaluronidase and Y-27632, a p160ROCK-specific inhibitor, to test the role of pericellular hyaluronan and Rho-ROCK activation in early and mature adhesion. Reduced adhesion resulted; greater inhibition of fibroblast adhesion was observed. We modulated the fibronectin affinity exhibited by both cell types using Nimesulide, an inhibitor of fibronectin integrin receptors alpha5beta1 and alphavbeta3. Our results are important in understanding vocal fold pathologies, wound healing, scarring, and in developing an accurate organotypic model of the vocal folds.

References

Feb 1, 1995·The Annals of Otology, Rhinology, and Laryngology·K Sato, M Hirano
Jan 1, 1996·The Annals of Otology, Rhinology, and Laryngology·A S PawlakS D Gray
Jul 1, 1996·The Annals of Otology, Rhinology, and Laryngology·M S CoureyR H Ossoff
Jun 16, 1999·The Laryngoscope·S D GrayT H Hammond
Dec 22, 1999·Biochemical and Biophysical Research Communications·N KawadaK Kaneda
Jan 29, 2000·The Annals of Otology, Rhinology, and Laryngology·S D GrayT H Hammond
Aug 5, 2000·Otolaryngologic Clinics of North America·S D Gray
Jan 24, 2002·Nature·Tomoyuki NakamuraKenneth R Chien
Apr 16, 2003·Journal of Biomechanics·Christina M Spofford, William M Chilian
Apr 19, 2003·Acta Oto-laryngologica·Kiminori SatoTadashi Nakashima
Jun 17, 2003·Cell Structure and Function·Mitsuru SatoHaruki Senoo
Jun 28, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hitoshi IkedaKenji Fujiwara
Oct 29, 2003·British Journal of Pharmacology·Atsushi MasamuneTooru Shimosegawa
Dec 17, 2003·Journal of Cell Science·Gerald Burgstaller, Mario Gimona
May 14, 2004·The Journal of the Acoustical Society of America·Ingo R Titze, Eric J Hunter
May 26, 2004·Biochemical Society Transactions·R Zaidel-BarB Geiger
Nov 13, 2004·The Annals of Otology, Rhinology, and Laryngology·Shigeru HiranoCharles N Ford
Jul 28, 2005·Cell and Tissue Research·Tannin J FujaIngo R Titze
Oct 29, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Tannin J FujaIngo R Titze

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Citations

Sep 22, 2010·Current Opinion in Otolaryngology & Head and Neck Surgery·Dinesh K Chhetri, Abie H Mendelsohn
Aug 8, 2009·Journal of Voice : Official Journal of the Voice Foundation·Keiko Ishikawa, Susan Thibeault
Jul 2, 2009·Journal of Biomedical Materials Research. Part a·Z FerdousK J Grande-Allen
Sep 12, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Christopher M RevellKyriacos A Athanasiou
Jun 29, 2012·The Annals of Otology, Rhinology, and Laryngology·Ingo R TitzeXiaoying Lu

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