PMID: 9523585Apr 2, 1998Paper

Differential cleavage of provasopressin by the major molecular forms of SPC3

Journal of Neurochemistry
L C Coates, N P Birch

Abstract

We have investigated the roles of full-length and carboxyl-terminus-truncated forms of the subtilisin-like prohormone convertase SPC3 in the processing of the radiolabeled vasopressin and oxytocin precursors, in vitro. We found SPC3 cleaves provasopressin at both the vasopressin-neurophysin and neurophysin-glycopeptide processing sites. Prooxytocin is cleaved by SPC3 at the oxytocin-neurophysin cleavage site. However, our results reveal differences in processing of provasopressin by the different molecular forms of SPC3. In incubations where the rate of autocatalytic carboxyl-terminus truncation of SPC3 was dramatically reduced, 86-kDa SPC3, which has an unprocessed carboxyl terminus, cleaved provasopressin at the neurophysin-glycopeptide junction. Cleavage at the vasopressin-neurophysin junction only occurred with the appearance of carboxyl-terminus-truncated forms of the enzyme. Incubations containing 64-kDa SPC3 or 64-kDa SPC3-T, a recombinant form of SPC3 truncated 14 amino acids beyond the conserved carboxyl-terminal "P-domain," rapidly cleaved provasopressin at both the vasopressin-neurophysin and neurophysin-glycopeptide junctions. Our results also suggest that prooxytocin is unable to be cleaved by the 86-kDa form of SP...Continue Reading

Citations

Mar 28, 2009·Critical Care Medicine·James A Russell
Feb 6, 2013·PloS One·Lindsay A PickettIris Lindberg
Apr 23, 2020·Journal of Neuroendocrinology·Mingkwan GreenwoodMichael Paul Greenwood
Apr 29, 2006·Human Molecular Genetics·David J LloydNicholas Gekakis
Aug 24, 2006·American Journal of Physiology. Endocrinology and Metabolism·Veronica Paez EspinosaTheodore C Friedman
Dec 9, 2004·American Journal of Physiology. Endocrinology and Metabolism·Xiaoxiong ShenTheodore C Friedman
Aug 3, 2004·The Journal of Clinical Investigation·Vanesa C SanchezEduardo A Nillni
May 18, 2016·Endocrine Reviews·Pieter StijnenJohn W M Creemers
Dec 4, 2001·Regulatory Peptides·M NakashimaT C Friedman
Dec 12, 2002·Chemical Reviews·Nathan C RockwellRobert S Fuller

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