Differential colocalization of profilin with microfilaments in PtK2 cells

Cell Motility and the Cytoskeleton
O MayborodaBrigitte M Jockusch

Abstract

Profilins are thought to be involved in the control of actin dynamics in eukaryotic cells. In accordance with this concept, profilin was found to be colocalized with the cortical microfilament webs in leading lamellae of locomoting and spreading fibroblasts. However, so far, there is little information on the distribution of profilin in other cell types. In this study, we report on the colocalization of profilin with various microfilament suprastructures in the epithelial cell line PtK2. This cell line, which is derived from rat kangaroo, contains a profilin sharing an N-terminal epitope with bovine and human profilin I, as seen by immunoblotting with monoclonal antibodies. By using immunofluorescence in conjunction with conventional fluorescence microscopy and confocal laser-scanning microscopy, we found profilin in ruffling areas of the peripheral lamellae and nascent stress fibers of spreading cells, whereas the peripheral belts of stationary cells growing in epithelioid sheets lacked profilin staining. In these cells, profilin was primarily distributed in a fine reticular or vesicular network that was not related to the microfilament system. Conspicuously low levels of profilins was not related to the contractile ring of mi...Continue Reading

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