Differential contribution of p300 and CBP to regulatory element acetylation in mESCs.

BMC Molecular and Cell Biology
Sara MartireLaura Banaszynski

Abstract

The transcription coactivators CREB binding protein (CBP) and p300 are highly homologous acetyltransferases that mediate histone 3 lysine 27 acetylation (H3K27ac) at regulatory elements such as enhancers and promoters. Although in most cases, CBP and p300 are considered to be functionally identical, both proteins are indispensable for development and there is evidence of tissue-specific nonredundancy. However, characterization of chromatin and transcription states regulated by each protein is lacking. In this study we analyze the individual contribution of p300 and CBP to the H3K27ac landscape, chromatin accessibility, and transcription in mouse embryonic stem cells (mESC). We demonstrate that p300 is the predominant H3K27 acetyltransferase in mESCs and that loss of acetylation in p300KD mESCs is more pronounced at enhancers compared to promoters. While loss of either CBP or p300 has little effect on the open state of chromatin, we observe that distinct gene sets are transcriptionally dysregulated upon depletion of p300 or CBP. Transcriptional dysregulation is generally correlated with dysregulation of promoter acetylation upon depletion of p300 (but not CBP) and appears to be relatively independent of dysregulated enhancer ace...Continue Reading

References

Sep 23, 1998·Genes & Development·K SakaguchiE Appella
Jun 14, 2006·Behavioral Neuroscience·Ana M M OliveiraMarcelo A Wood
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jan 30, 2010·Bioinformatics·Aaron R Quinlan, Ira M Hall
May 4, 2010·Nucleic Acids Research·Yolande F M RamosPeter A C 't Hoen
Jan 12, 2011·Nature Biotechnology·James T RobinsonJill P Mesirov
Aug 6, 2011·Genome Research·Lichun JiangBrian Oliver
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Oct 15, 2013·Cell·Denes HniszRichard A Young
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Oct 18, 2014·ACS Chemical Biology·Ryan A HenryAndrew J Andrews
Jan 17, 2015·Chemical Reviews·Beverley M Dancy, Philip A Cole
Apr 16, 2016·Nucleic Acids Research·Fidel RamírezThomas Manke
Jan 24, 2017·Cell·Constantinos ChronisKathrin Plath
Oct 4, 2017·Nucleic Acids Research·Scott T Younger, John L Rinn
Aug 16, 2018·Cell Reports·Ryan RaisnerKaren E Gascoigne

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Methods Mentioned

BETA
histone acetylation
acetylation
ChIP-seq
immunoprecipitation
RNA-seq
acetylations
ChIPs
Assay
PCR
ChIP-

Software Mentioned

MACS14
UpSet Plots
BEDTools
FastQC
UCSC genome browser
count
R
DeepTools
multiBamSummary
HOMER

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