Differential contribution of skeletal and cardiac II-III loop sequences to the assembly of dihydropyridine-receptor arrays in skeletal muscle

Molecular Biology of the Cell
H TakekuraBernhard E Flucher

Abstract

The plasmalemmal dihydropyridine receptor (DHPR) is the voltage sensor in skeletal muscle excitation-contraction (e-c) coupling. It activates calcium release from the sarcoplasmic reticulum via protein-protein interactions with the ryanodine receptor (RyR). To enable this interaction, DHPRs are arranged in arrays of tetrads opposite RyRs. In the DHPR alpha(1S) subunit, the cytoplasmic loop connecting repeats II and III is a major determinant of skeletal-type e-c coupling. Whether the essential II-III loop sequence (L720-L764) also determines the skeletal-specific arrangement of DHPRs was examined in dysgenic (alpha(1S)-null) myotubes reconstituted with distinct alpha(1) subunit isoforms and II-III loop chimeras. Parallel immunofluorescence and freeze-fracture analysis showed that alpha(1S) and chimeras containing L720-L764, all of which restored skeletal-type e-c coupling, displayed the skeletal arrangement of DHPRs in arrays of tetrads. Conversely, alpha(1C) and those chimeras with a cardiac II-III loop and cardiac e-c coupling properties were targeted into junctional membranes but failed to form tetrads. However, an alpha(1S)-based chimera with the heterologous Musca II-III loop produced tetrads but did not reconstitute skele...Continue Reading

References

Jun 1, 1977·The Journal of Physiology·M Hencek, J Zachar
Sep 1, 1984·The Journal of General Physiology·W F Gilly, T Scheuer
Jun 1, 1995·Journal of Muscle Research and Cell Motility·C Franzini-Armstrong, J W Kish
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·H TakekuraC Franzini-Armstrong
May 1, 1995·The Journal of Cell Biology·X H SunC Franzini-Armstrong
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·A AraqueW Buño
Aug 1, 1994·Biophysical Journal·H TakekuraC Franzini-Armstrong
Jan 10, 1996·Developmental Biology·F ProtasiC Franzini-Armstrong
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·M GrabnerK G Beam
Mar 21, 1998·The Journal of Cell Biology·F ProtasiP D Allen
Oct 29, 2000·Biophysical Journal·F ProtasiC Franzini-Armstrong
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·C M WilkensM Grabner
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·C A AhernR Coronado
Dec 1, 2001·The Journal of Biological Chemistry·Catherine ProenzaKurt G Beam
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Bernhard E FlucherManfred Grabner

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Citations

Apr 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anamika DayalManfred Grabner
Jun 30, 2010·The Journal of General Physiology·Kurt G Beam, Roger A Bannister
Dec 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Weijun ChengRoberto Coronado
Nov 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Johann SchredelsekerManfred Grabner
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Petronel TulucBernhard E Flucher
Dec 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·David C SheridanClaudio F Perez
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Joseph W SangerJean M Sanger
Nov 16, 2005·The Journal of Cell Biology·Valentina Di Biase, Clara Franzini-Armstrong
Sep 18, 2012·Biochimica Et Biophysica Acta·Roger A Bannister, Kurt G Beam
Jan 1, 2014·The International Journal of Biochemistry & Cell Biology·Robyn T RebbeckAngela F Dulhunty
Oct 4, 2016·Clinical and Experimental Pharmacology & Physiology·Angela F DulhuntyNicole A Beard
Dec 30, 2017·The Journal of General Physiology·Alexander PolsterSymeon Papadopoulos
Jan 24, 2017·Scientific Reports·Marta Campiglio, Bernhard E Flucher
Aug 3, 2018·Journal of Cellular Physiology·Marta CampiglioBernhard E Flucher
Sep 28, 2007·Journal of Muscle Research and Cell Motility·Roger A Bannister
Oct 6, 2009·Journal of Muscle Research and Cell Motility·R A Bannister, K G Beam
Aug 10, 2005·Journal of Muscle Research and Cell Motility·Bernhard E FlucherManfred Grabner
Oct 14, 2011·Journal of Muscle Research and Cell Motility·Christopher L-H HuangJames A Fraser
Mar 31, 2020·Pflügers Archiv : European journal of physiology·Bernhard E Flucher
Jan 9, 2019·Frontiers in Physiology·Peter P JonesWilliam E Louch
Dec 13, 2017·Proceedings of the National Academy of Sciences of the United States of America·Stefano PerniKurt G Beam
Dec 23, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jeremy W LinsleyJohn Y Kuwada
Mar 1, 2013·Journal of Cell Science·Marta CampiglioBernhard E Flucher

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