Differential control of 17 alpha-hydroxylase and 3 beta-hydroxysteroid dehydrogenase expression in human adrenocortical H295R cells

The Journal of Clinical Endocrinology and Metabolism
I M BirdJ I Mason

Abstract

Previous studies of human adrenocortical cells have given inconsistent findings concerning the effects of angiotensin II (AII) alone or in combination with activators of the protein kinase A-signaling pathway on expression of cholesterol side-chain cleavage cytochrome P450 (P450scc), 17 alpha-hydroxylase cytochrome P450 (P450c17), and 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), as well as the corresponding effects on adrenocortical cell steroid secretory products. We have used the human adrenocortical carcinoma H295R cell to evaluate further this question and determine the role of protein kinase C in each of these responses to AII. Treatment with AII alone (10 nmol/L, 48 h) resulted in a significant increase in cortisol production (1.8-fold), as well as a much greater effect on aldosterone production. This increased formation of 17 alpha-hydroxysteroids was accompanied by increased expression of P450c17 as determined at the level of messenger RNA (mRNA) and enzyme activity. Similar increases in expression of P450scc were observed at the level of mRNA. Increases in 3 beta-HSD expression were also seen at the level of mRNA and, to a lesser extent, at the level of enzyme activity. Because of the comparatively low basal 17 al...Continue Reading

Citations

Nov 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·M Ehrhart-BornsteinS R Bornstein
Aug 16, 2011·Molecular and Cellular Endocrinology·Namita G HattangadyWilliam E Rainey
Nov 16, 2004·Molecular and Cellular Endocrinology·William E RaineyBernard P Schimmer
Oct 16, 1999·The Journal of Clinical Endocrinology and Metabolism·A ChakravortyR B Jaffe
Mar 25, 2017·Frontiers in Endocrinology·Xavier Bertagna
Aug 3, 2021·Frontiers in Endocrinology·Arthur D ZimmermanChad D Foradori

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Adrenocortical carcinoma (ACC) is a rare malignancy of the adrenal cortex, associated with a generally dismal prognosis owing to its aggressive behavior. Here are the latest discoveries pertaining to this disease.