Differential correlations between changes to glutathione redox state, protein ubiquitination, and stress-inducible HSPA chaperone expression after different types of oxidative stress

Cell Stress & Chaperones
Pierre-Marie GirardJean-Marc Lelièvre

Abstract

In primary bovine fibroblasts with an hspa1b/luciferase transgene, we examined the intensity of heat-shock response (HSR) following four types of oxidative stress or heat stress (HS), and its putative relationship with changes to different cell parameters, including reactive oxygen species (ROS), the redox status of the key molecules glutathione (GSH), NADP(H) NAD(H), and the post-translational protein modifications carbonylation, S-glutathionylation, and ubiquitination. We determined the sub-lethal condition generating the maximal luciferase activity and inducible HSPA protein level for treatments with hydrogen peroxide (H2O2), UVA-induced oxygen photo-activation, the superoxide-generating agent menadione (MN), and diamide (DA), an electrophilic and sulfhydryl reagent. The level of HSR induced by oxidative stress was the highest after DA and MN, followed by UVA and H2O2 treatments, and was not correlated to the level of ROS production nor to the extent of protein S-glutathionylation or carbonylation observed immediately after stress. We found a correlation following oxidative treatments between HSR and the level of GSH/GSSG immediately after stress, and the increase in protein ubiquitination during the recovery period. Convers...Continue Reading

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Citations

Nov 3, 2021·Journal of Biochemical and Molecular Toxicology·Ting LiuJiang Zheng

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Methods Mentioned

BETA
transgenic
Protein Oxidation
electrophoresis
ubiquitination
FACS
FCS
PCR
flow cytometry
carbonylation
polyubiquitination

Software Mentioned

qbasePLUS
[UNK]
SigmaPlot
ImageJ
qBase plus
R

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