Differential coupling of alpha1-adrenoreceptor subtypes to phospholipase C and mitogen activated protein kinase in neonatal rat cardiac myocytes

European Journal of Pharmacology
D WenhamJ D Robishaw

Abstract

Activation of cardiac alpha1-adrenoreceptors has a number of physiological effects. Ascribing these effects to a specific alpha1-adrenoreceptor subtype first requires the elucidation of the subtypes that are present in the tissue of interest. In the present study, mRNA transcripts for the alpha1A, alpha1B and alpha1D-adrenoreceptor subtypes were detected in cultured neonatal rat cardiac myocytes, using reverse transcriptase-polymerase chain reaction analysis. However, binding sites for only the alpha1A and alpha1B-adrenoreceptor subtypes were detected in cultured neonatal rat cardiac myocytes, using competition binding analysis with a variety of alpha1 selective receptor antagonists. Phenylephrine-stimulated phosphatidylinositol hydrolysis was inhibited by alpha1 selective receptor antagonists with affinities consistent with the alpha1A-adrenoreceptor subtype, whereas phenylephrine-induced activation of the mitogen activated protein kinase cascade was inhibited by these same antagonists with affinities more closely resembling the alpha1B-adrenoreceptor subtype. In the case of both signaling pathways, the alpha1D selective receptor antagonist, BMY 7378, exhibited affinities suggestive of the relative absence of a alpha1D-adrenor...Continue Reading

References

Jan 1, 1991·Circulation·H E Morgan, K M Baker
Feb 25, 1990·Nucleic Acids Research·M M VoigtH M Chin
Aug 23, 1990·Nature·J DownwardD A Cantrell
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S CotecchiaB K Kobilka
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
Apr 1, 1995·Bioscience Reports·A G Gilman
Jan 16, 1995·European Journal of Pharmacology·A S GoetzD L Saussy
Jan 1, 1995·Journal of Molecular and Cellular Cardiology·C A HansenJ D Robishaw
Dec 30, 1994·Biochimica Et Biophysica Acta·C A HansenJ D Robishaw
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·W J KochR J Lefkowitz
Feb 1, 1994·British Journal of Pharmacology·M C MichelG Gross
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·C A MilanoR J Lefkowitz
Aug 1, 1994·Naunyn-Schmiedeberg's Archives of Pharmacology·M C Michel, P A Insel
Apr 1, 1993·Trends in Pharmacological Sciences·P Leff, I G Dougall
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·D D D'AngeloG W Dorn
Sep 1, 1995·Trends in Cardiovascular Medicine·E O Harrington, J A Ware

❮ Previous
Next ❯

Citations

Dec 19, 2001·Journal of Cellular Physiology·K SinghW S Colucci
Jun 22, 2000·Journal of Molecular and Cellular Cardiology·S RohdeS F Steinberg
Apr 19, 2002·Journal of Neuroimmunology·Cobi J HeijnenAnnemieke Kavelaars
Mar 30, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Ravi GoyalLawrence D Longo
Feb 20, 2002·Journal of Molecular and Cellular Cardiology·M Isabel Tejero-TaldoRakesh C Kukreja
Mar 1, 2000·Journal of Neuroimmunology·C J Heijnen, A Kavelaars
Jan 28, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Marie-Josée BenoitBruce G Allen
Feb 22, 2001·American Journal of Physiology. Renal Physiology·F Liu, F A Gesek
Sep 20, 2000·American Journal of Physiology. Heart and Circulatory Physiology·B H WangE A Woodcock
Jul 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·I LemireH Rindt
Feb 23, 2011·Journal of Receptor and Signal Transduction Research·Dianne M Perez, Van A Doze
Jul 27, 2001·Archives of Biochemistry and Biophysics·S SeraskerisA Lazou
Dec 28, 1999·European Journal of Pharmacology·C González-EspinosaJ A García-Sáinz
Aug 12, 1999·European Journal of Pharmacology·H Zhong, K P Minneman
Oct 24, 2002·Journal of Molecular and Cellular Cardiology·Susan F Steinberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.