Differential cytokine mRNA expression in mice after oral exposure to the trichothecene vomitoxin (deoxynivalenol): dose response and time course

Toxicology and Applied Pharmacology
H R ZhouJ J Pestka

Abstract

Acute oral exposure of B6C3F1 mice to vomitoxin (VT) has been previously shown to induce expression of mRNAs for cytokines that are characteristically produced in lymphoid tissues by macrophage and T cells. The purpose of this study was to evaluate the effects of VT dose on the expression of mRNAs for a cytokine profile consisting of TNF-alpha, IL-1beta, IL-6, IL-12, IFN-gamma, IL-2, IL-4, IL-5, IL-10, IL-12, and TGF-beta and to measure the kinetics of these responses. The effects of a single oral exposure to 0, 0.1, 0.5, 1, 5, and 25 mg/kg BW of VT on cytokine mRNA abundance in spleen and Peyer's patches (indicators of systemic and mucosal immune compartments, respectively) were assessed at 2 hr postexposure using reverse transcriptase-polymerase chain reaction (RT-PCR) in combination with hybridization analysis. Both 5 and 25 mg/kg VT significantly induced the mRNAs for the proinflammatory cytokines IL-1beta, IL-6, and TNF-alpha; the T helper 1 cytokines IFN-gamma and IL-2; and the T helper 2 cytokines IL-4 and IL-10, whereas lower doses had no effect. IL-12p40 mRNA was also induced but not IL-12p35 mRNA. The effects were more pronounced in spleen than in the Peyer's patches. IL-5 and TGF-beta mRNAs were expressed constitutiv...Continue Reading

Citations

Feb 25, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·J J Pestka
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Aug 9, 2002·Journal of Toxicology and Environmental Health. Part a·Shu-Shyan WongJames J Pestka
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May 30, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hui-Ren ZhouJames J Pestka
Jul 25, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Chidozie J AmuzieJames J Pestka
Oct 7, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Chidozie J Amuzie, James J Pestka
Aug 21, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Brenna M FlanneryJames J Pestka
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Jun 25, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hui-Ren ZhouJames J Pestka

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