Differential cytokine regulation of natural killer cell-mediated necrotic and apoptotic cytotoxicity

Immunology
C M Gardiner, D J Reen

Abstract

Natural killer (NK) cells can kill target cells by either necrotic or apoptotic mechanisms. Using the 51Cr-release assay to measure necrotic death of target cells, neonatal NK cells had low NK activity (K562 targets) and high lymphokine-activated killer (LAK) activity (Daudi targets) compared with adult cells, as has been previously reported. Using a 125I-deoxyuridine (125I-UdR) release assay, cord cells were shown to also have higher apoptotic LAK activity against YAC-1 target cells. Interleukin-4 (IL-4) inhibited interleukin-2 (IL-2)-induced necrotic killing of target cells by adult effectors but had no such inhibitory effect on cord cells. In contrast, IL-4 inhibited both adult and cord LAK cytotoxicity of YAC-1 target cells by apoptotic mechanisms with higher suppression observed in cord cell preparations. Using a colorimetric substrate conversion assay, IL-2 induced higher, and IL-4 had a more significant suppressive effect on, cord cell granzyme B enzyme activity compared with adult cells, paralleling apoptosis cytotoxicity data. Co-culture of either adult or cord LAK cells with IL-4 had a similar inhibitory effect on granzyme B protein expression, as detected by Western blotting. In contrast, IL-4 did not inhibit perfori...Continue Reading

References

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Citations

Nov 11, 2017·American Journal of Rhinology & Allergy·Sarah E SmithJennifer K Mulligan
Aug 18, 1999·Immunological Reviews·O T ChanM J Shlomchik
Jul 23, 2009·Acta Microbiologica Et Immunologica Hungarica· SuyarsonoWihaskoro Sosroseno
Aug 19, 2020·Journal of Clinical Laboratory Analysis·Dan ZhangJinsong Hu
Apr 25, 2003·Alcoholism, Clinical and Experimental Research·Madhavi DokurDipak K Sarkar

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