Differential cytolysis of murine spleen, bone-marrow and leukemia cells by melittin reveals differences in membrane topography

Biochemical and Biophysical Research Communications
J J Killion, J D Dunn

Abstract

L1210 leukemia cells are 2-4 fold more sensitive to the cytolytic effects of melittin, the membrane-active toxin of bee venom, than normal DBA/2 mouse spleen and bone-marrow cells. Lysis of the normal cells was abolished when either 75 mM galactosamine, glucosamine or 100 microM beta-lactoglobulin was added to the melittin-cell reaction, but lysis of the leukemia cells was unaffected. The amino-groups appeared necessary for blocking melittin-mediated lysis since glucose, galactose and the N-acetyl derivatives were not inhibitory. Bone-marrow cells were more readily protected from lysis than spleen cells. Since melittin-inhibitor complexes were not detected by gel chromatography and the inhibitor could be added to the cell suspension after melittin, the evidence suggests that bone-marrow cells are rich in membrane binding sites for carbohydrates that decrease in mature spleen cells and are virtually absent after neoplastic transformation.

References

Jul 16, 1985·Biochemical and Biophysical Research Communications·D A Malencik, S R Anderson
Jan 1, 1985·The Journal of Membrane Biology·M T TostesonD C Tosteson
Jul 28, 1972·Science·E Habermann
May 1, 1954·Experimental Cell Research·M ABERCROMBIE, J E HEAYSMAN
Apr 1, 1961·Proceedings of the Society for Experimental Biology and Medicine·K HABEL

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Citations

Nov 24, 2011·Cancer Metastasis Reviews·Nada Oršolić
Feb 5, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Adrian Florea, Constantin Crăciun
Oct 1, 2009·Arhiv za higijenu rada i toksikologiju·Nada Orsolić
Dec 1, 2003·Veterinary and Comparative Oncology·N OrsolićI Basić
Sep 23, 2011·Biochemical Society Transactions·Katrijn De BruckerKarin Thevissen
Jul 31, 2013·Environmental Toxicology and Pharmacology·Goran Gajski, Vera Garaj-Vrhovac
Dec 15, 2007·Biochimica Et Biophysica Acta·David W Hoskin, Ayyalusamy Ramamoorthy
Jan 1, 1988·Annals of the New York Academy of Sciences·B K LamP Y Wong
Sep 2, 2009·Anais Da Academia Brasileira De Ciências·Elaine G RodriguesLuiz R Travassos
Jan 6, 2021·International Journal of Molecular Sciences·Teresa KreinestMartin S Staege
Jun 1, 1997·Infection and Immunity·E M PorterT Ganz

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