PMID: 6198312Dec 1, 1983Paper

Differential cytotoxicity of cis-platinum and blenoxane against human carcinoma KBE cells in multicellular spheroids of different ages: response at different temperatures

International Journal of Radiation Oncology, Biology, Physics
M L MeltzT C Pomeroy


Human KBE epidermoid carcinoma cells were reproducibly grown in suspension as multicellular spheroids (MCS). Initial aggregation at 48 hours is followed by a rapid diameter increase until day 10. The size increase then continues with daily refeedings, under the growth conditions used, but at a slower rate. When cells are treated in MCS with either blenoxane (bleomycin) or cis-diamminedichloroplatinum (II) (cis-platinum), the survival of cells (by cloning efficiency (CE) essay) varied from that of surface attached (SA) cells. The survival was dependent on the age of the MCS as well as their size; the age response was significantly different for cis-platinum and blenoxane. Hyperthermic incubation of KBE cells in MCS at different ages for 1 hour (40-43 degrees C) resulted in cell killing similar to that observed after hyperthermic incubation of surface attached cells. In combined hyperthermia/chemotherapy experiments, simultaneous treatment with blenoxane resulted in little or no increase in MCS cell toxicity at 40 degrees C; at 42.5 degrees C, there was increased toxicity. The increase in toxicity was similar for MCS of different ages. Upon simultaneous cis-platinum treatment, an increase in toxicity was observed at 40 degrees C,...Continue Reading


Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G M HahnI Har-Kedar
May 1, 1977·Radiology·W C DeweyL E Gerweck
Apr 1, 1978·The British Journal of Radiology·F A Stewart, J Denekamp
Mar 1, 1973·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R M Sutherland, R E Durand
Jul 1, 1974·Cancer·H D Suit, M Shwayder
Feb 1, 1972·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K Overgaard, J Overgaard
Jan 1, 1980·Annals of the New York Academy of Sciences·W C Dewey, M L Freeman
Jul 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·H EAGLE

❮ Previous
Next ❯


Jan 1, 1987·Journal of Cancer Research and Clinical Oncology·W Mueller-Klieser

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Related Papers

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
M MareelL De Ridder
Zeitschrift Für Krebsforschung Und Klinische Onkologie. Cancer Research and Clinical Oncology
A SchleichA Mayer
© 2021 Meta ULC. All rights reserved