Differential deposition of fibronectin by asthmatic bronchial epithelial cells

American Journal of Physiology. Lung Cellular and Molecular Physiology
Qi GeJanette K Burgess

Abstract

Altered ECM protein deposition is a feature in asthmatic airways. Fibronectin (Fn), an ECM protein produced by human bronchial epithelial cells (HBECs), is increased in asthmatic airways. This study investigated the regulation of Fn production in asthmatic or nonasthmatic HBECs and whether Fn modulated HBEC proliferation and inflammatory mediator secretion. The signaling pathways underlying transforming growth factor (TGF)-β1-regulated Fn production were examined using specific inhibitors for ERK, JNK, p38 MAPK, phosphatidylinositol 3 kinase, and activin-like kinase 5 (ALK5). Asthmatic HBECs deposited higher levels of Fn in the ECM than nonasthmatic cells under basal conditions, whereas cells from the two groups had similar levels of Fn mRNA and soluble Fn. TGF-β1 increased mRNA levels and ECM and soluble forms of Fn but decreased cell proliferation in both cells. The rate of increase in Fn mRNA was higher in nonasthmatic cells. However, the excessive amounts of ECM Fn deposited by asthmatic cells after TGF-β1 stimulation persisted compared with nonasthmatic cells. Inhibition of ALK5 completely prevented TGF-β1-induced Fn deposition. Importantly, ECM Fn increased HBEC proliferation and IL-6 release, decreased PGE2 secretion, bu...Continue Reading

References

Jun 1, 1992·Molecular Reproduction and Development·H L Moses
Mar 11, 1989·Lancet·W R RocheS T Holgate
May 1, 1995·International Archives of Allergy and Immunology·J BousquetF B Michel
Feb 18, 1995·Lancet·I D Pavord, A E Tattersfield
Mar 1, 1995·Chest·C R Roberts
Jul 1, 1993·The American Review of Respiratory Disease·I D PavordA E Tattersfield
Feb 3, 1997·The Journal of Experimental Medicine·M RincónR A Flavell
Aug 1, 1997·American Journal of Respiratory and Critical Care Medicine·A M VignolaJ Bousquet
Sep 22, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·M K Magnusson, D F Mosher
Dec 16, 1998·American Journal of Respiratory and Critical Care Medicine·H W ChuS E Wenzel
Mar 11, 2000·American Journal of Respiratory and Critical Care Medicine·J A Elias
Apr 1, 2000·The Journal of Biological Chemistry·D C HockingK J Langenbach
Sep 6, 2000·American Journal of Respiratory Cell and Molecular Biology·S J HirstT H Lee
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S D GriegoS B Dillon
Dec 9, 2000·American Journal of Respiratory and Critical Care Medicine·P R JohnsonP A Underwood
Aug 10, 2002·The Journal of Allergy and Clinical Immunology·Hironori SagaraAtsuhito Nakao
Mar 18, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Denise C Hocking, Cecilia H Chang
May 20, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Linda S ChambersJanette K Burgess
Apr 30, 2005·Thorax·I A FedorovS T Holgate
Jun 11, 2005·Respiratory Research·Hidenori KasaiZhi Zhang
Aug 9, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Masaharu ShinkaiBruce K Rubin
Aug 16, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Janette K BurgessPeter R A Johnson
Sep 24, 2005·American Journal of Respiratory and Critical Care Medicine·Peter R A JohnsonJudith L Black
Aug 16, 2006·American Journal of Respiratory and Critical Care Medicine·Anthony KicicStephen M Stick
Mar 27, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Grzegorz SkibinskiMadeleine Ennis
May 1, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shaoping XieKian Fan Chung
Sep 18, 2007·American Journal of Respiratory Cell and Molecular Biology·Abdelhabib SemlaliJamila Chakir
Jan 30, 2010·American Journal of Respiratory and Critical Care Medicine·Anthony KicicDarryl A Knight
Jul 21, 2011·Current Allergy and Asthma Reports·Yui-Hsi Wang, Marsha Wills-Karp
Jul 23, 2011·American Journal of Respiratory Cell and Molecular Biology·David I KrimmerBrian G G Oliver
Mar 2, 2013·American Journal of Respiratory Cell and Molecular Biology·Tjitske A OenemaBart G J Dekkers

❮ Previous
Next ❯

Citations

Jun 1, 2016·Journal of Pharmaceutical and Biomedical Analysis·P FerroJ Segura
Aug 14, 2018·Cellular and Molecular Life Sciences : CMLS·Marta MichalikZbigniew Madeja
Jan 12, 2021·Frontiers in Bioengineering and Biotechnology·Mirko D'Urso, Nicholas A Kurniawan
Aug 9, 2021·Molecular Aspects of Medicine·Zsolt I KomlósiCezmi A Akdis
Aug 26, 2021·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Soyoon SimHae-Sim Park

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.