Differential Diagnosis of Malaria on Truelab Uno®, a Portable, Real-Time, MicroPCR Device for Point-Of-Care Applications

PloS One
Chandrasekhar Bhaskaran NairSusanta K Ghosh

Abstract

Sensitive and specific detection of malarial parasites is crucial in controlling the significant malaria burden in the developing world. Also important is being able to identify life threatening Plasmodium falciparum malaria quickly and accurately to reduce malaria related mortality. Existing methods such as microscopy and rapid diagnostic tests (RDTs) have major shortcomings. Here, we describe a new real-time PCR-based diagnostic test device at point-of-care service for resource-limited settings. Truenat® Malaria, a chip-based microPCR test, was developed by bigtec Labs, Bangalore, India, for differential identification of Plasmodium falciparum and Plasmodium vivax parasites. The Truenat Malaria tests runs on bigtec's Truelab Uno® microPCR device, a handheld, battery operated, and easy-to-use real-time microPCR device. The performance of Truenat® Malaria was evaluated versus the WHO nested PCR protocol. The Truenat® Malaria was further evaluated in a triple-blinded study design using a sample panel of 281 specimens created from the clinical samples characterized by expert microscopy and a rapid diagnostic test kit by the National Institute of Malaria Research (NIMR). A comparative evaluation was done on the Truelab Uno® and a ...Continue Reading

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Sep 20, 2017·Diagnostics·Francis D KrampaProsper Kanyong
Nov 11, 2017·Lab on a Chip·N KolluriM Cabodi
Feb 12, 2020·Biotechnology Journal·Shipra SolankiBansi D Malhotra
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Jul 6, 2021·Frontiers in Cellular and Infection Microbiology·Alba Marina GimenezDaniel Youssef Bargieri
Oct 17, 2020·Omics : a Journal of Integrative Biology·Georgios PsariasGeorge P Patrinos

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Methods Mentioned

BETA
PCR
chip
electrophoresis
chips
sandwich immunoassay
amplicon purification
nucleic acid amplification
nucleic acid extraction

Software Mentioned

Truelab Uno
Truelab
Trueprep MAG
ClustalW
Truenat
bigtec

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