Differential Disease Susceptibilities in Experimentally Reptarenavirus-Infected Boa Constrictors and Ball Pythons

Journal of Virology
Mark D StengleinJoseph L DeRisi

Abstract

Inclusion body disease (IBD) is an infectious disease originally described in captive snakes. It has traditionally been diagnosed by the presence of large eosinophilic cytoplasmic inclusions and is associated with neurological, gastrointestinal, and lymphoproliferative disorders. Previously, we identified and established a culture system for a novel lineage of arenaviruses isolated from boa constrictors diagnosed with IBD. Although ample circumstantial evidence suggested that these viruses, now known as reptarenaviruses, cause IBD, there has been no formal demonstration of disease causality since their discovery. We therefore conducted a long-term challenge experiment to test the hypothesis that reptarenaviruses cause IBD. We infected boa constrictors and ball pythons by cardiac injection of purified virus. We monitored the progression of viral growth in tissues, blood, and environmental samples. Infection produced dramatically different disease outcomes in snakes of the two species. Ball pythons infected with Golden Gate virus (GoGV) and with another reptarenavirus displayed severe neurological signs within 2 months, and viral replication was detected only in central nervous system tissues. In contrast, GoGV-infected boa const...Continue Reading

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Citations

Mar 29, 2019·Australian Veterinary Journal·T H HyndmanS D Vitali
Sep 10, 2019·PloS One·Katharina WindbichlerJussi Hepojoki
Jan 26, 2020·Animals : an Open Access Journal From MDPI·Jennah GreenNeil D'Cruze
Mar 27, 2020·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Janosch DietzRachel E Marschang
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Mar 29, 2019·Frontiers in Immunology·Morgan E Brisse, Hinh Ly
Jan 15, 2021·Journal of Virology·U HetzelJ Hepojoki
Apr 25, 2021·The Veterinary Clinics of North America. Exotic Animal Practice·Rachel E MarschangMichael Pees

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Datasets Mentioned

BETA
PRJNA383000

Methods Mentioned

BETA
biopsy
reverse transcription-PCR
fluorescence microscopy
Necropsy
PCR

Software Mentioned

Zeiss
Zen Microscopy
ImageJ

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