PMID: 9171157May 1, 1997Paper

Differential dissection of the rat E16 ventral mesencephalon and survival and reinnervation of the 6-OHDA-lesioned striatum by a subset of aldehyde dehydrogenase-positive TH neurons

Cell Transplantation
N S HaqueO Isacson

Abstract

The retinoic acid-generating enzyme, aldehyde dehydrogenase (AHD), is expressed in a subpopulation of dopaminergic neurons found in the substantia nigra. Using AHD and tyrosine hydroxylase (TH) as immunohistochemical markers, we determined whether differential dissection of the embryonic (E16) ventral mesencephalon (VM) into its lateral and medial portions contributed equally to the number of TH cells surviving transplantation, if grafted AHD/TH neurons reinnervate the host striatum according to their normal projection patterns, and examined the functional recovery caused by the implanted cells as assessed by amphetamine-induced rotation in a 6-OHDA-lesioned model of Parkinson's disease. The embryonic tissue was transplanted as solid pieces injected via a 20-gauge lumbar puncture needle into the center of the deafferented striatum. Groups received either one complete ventral mesencephalic piece (VM), two medial pieces of ventral mesencephalic tissue (MVM), or two lateral pieces of ventral mesencephalic tissue (LVM). Both VM and MVM groups showed a significant decrease in amphetamine-induced rotation over time and, there was no difference in the degree of reduction observed between the two groups. Histological evaluation of the ...Continue Reading

Citations

Aug 22, 2001·Microscopy Research and Technique·I StrömbergP M Almqvist
Mar 5, 2004·Expert Opinion on Biological Therapy·Andreas HermannAlexander Storch
Nov 4, 2000·Expert Opinion on Investigational Drugs·C V Borlongan
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lars M BjorklundOle Isacson
Dec 5, 2009·Current Protocols in Stem Cell Biology·Jan PruszakGuido Nikkhah
Mar 5, 2014·BioMed Research International·B WeissO Schmitt
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Olle Lindvall, Anders Björklund

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