Differential distribution of glutamylated tubulin isoforms along the sea urchin sperm axoneme

Molecular Reproduction and Development
P HuitorelC Gagnon

Abstract

Tubulin belongs to a highly conserved multigenic family, in which several gene products usually coexist in the same tissue or the same cell. Moreover, seven classes of post-translational modifications of these gene products lead to an amazing diversity of tubulin polypeptide chains, within the same cell type, whose physiological function remains elusive. Such diversity has been found in a very stable microtubular organelle, the sea urchin sperm flagellum, where some tubulin isoforms have been directly implicated in motility, whereas others may play a more structural role. In particular, polyglutamylated tubulin has been shown to be crucial for motility (Gagnon et al., 1996: J Cell Sci 109:1545 p). Here, we show with the GT335 antibody that polyglutamylated tubulin is distributed according to a decreasing gradient along the sea urchin sperm axoneme, since a semi-quantitative measurement of immunofluorescence intensity reveals that in its proximal half, the axoneme is sixfold more labeled than in its distal half. This gradient along the length of the axoneme is confirmed by immunogold labeling procedures which, in addition, demonstrate a uniform distribution of polyglutamylated tubulin among peripheral doublets and a lesser conte...Continue Reading

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Citations

Nov 19, 2004·Parasitology Research·Uwe PlessmannKarl-Ferdinand Lechtreck
May 19, 2011·Molecular Human Reproduction·Kazuo Inaba
Oct 13, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Hideo MohriShoji A Baba
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May 6, 2019·International Journal of Molecular Sciences·Maria Antonietta RagusaFabrizio Gianguzza
Aug 15, 2009·Journal of Proteome Research·Chying-Chyuan ChanGwo-Jang Wu

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