Differential distribution of non-structural proteins of foot-and-mouth disease virus in BHK-21 cells

Virology
Mercedes García-BrionesRosario Armas-Portela

Abstract

Differences in the kinetics of expression and cell distribution among FMDV non-structural proteins (NSPs) have been observed in BHK-21-infected cells. 3D(pol) was the first protein detected by immunofluorescence (1.5 h p.i.), showing a perinuclear distribution. At 2-2.5 h p.i., 2B, 2C, 3B and 3C were detected, mostly exhibiting a punctuated, scattered pattern, while 3A and 3D(pol) appeared concentrated at one side of the nucleus. This distribution was exhibited by all the NSPs from 3 h p.i., being 2C and, to a lesser extent, precursors 2BC and 3ABBB, the only proteins detected by Western blotting at that infection time. From 4 h p.i., all mature NSPs as well as precursors 2BC, 3ABBB, 3ABB, 3AB and 3CD(pol) were detected by this technique. In spite of their similar immunofluorescence patterns, 2C and 3A co-localized partially by confocal microscopy at 3.5 h p.i., and 3A, but not 2C, co-localized with the ER marker calreticulin, suggesting differences in the distribution of these proteins and/or their precursors as infection proceeded. Transient expression of 2C and 3AB resulted in punctuated fluorescence patterns similar to those found in early infected cells, while 3A showed a more diffuse distribution. A shift towards a fibrou...Continue Reading

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Citations

Oct 4, 2015·The Journal of General Virology·Morgan R HerodNicola J Stonehouse
Apr 6, 2013·The Journal of General Virology·Julian SeagoTerry Jackson
Jul 28, 2013·Virology·M Teresa Sánchez-AparicioFrancisco Sobrino
Apr 21, 2007·Virology·Miguel Rodríguez PulidoEncarnación Martínez-Salas
Apr 11, 2008·FEMS Immunology and Medical Microbiology·Marvin J GrubmanTeresa de Los Santos
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Nov 30, 2018·Frontiers in Microbiology·Gisselle N MedinaTeresa de Los Santos
Sep 3, 2019·Frontiers in Microbiology·Patricia de LeónAngel L Carrascosa

Related Concepts

Cell Nucleus
Cytopathogenic Effect, Viral
Protoplasm
Endoplasmic Reticulum
Cricetus
Immunofluorescence Microscopy
Subcellular Fractions
Transfection
Western Blot
Viral Nonstructural Proteins NS2

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