Differential distribution of Shank and GKAP at the postsynaptic density

PloS One
Jung-Hwa Tao-ChengAyse Dosemeci

Abstract

Shank and GKAP are scaffold proteins and binding partners at the postsynaptic density (PSD). The distribution and dynamics of Shank and GKAP were studied in dissociated hippocampal cultures by pre-embedding immunogold electron microscopy. Antibodies against epitopes containing their respective mutual binding sites were used to verify the expected juxtapositioning of Shank and GKAP. If all Shank and GKAP molecules at the PSD were bound to each other, the distribution of label for the two proteins should coincide. However, labels for the mutual binding sites showed significant differences in distribution, with a narrow distribution for GKAP located close to the postsynaptic membrane, and a wider distribution for Shank extending deeper into the cytoplasm. Upon depolarization with high K+, neither the intensity nor distribution of label for GKAP changed, but labeling intensity for Shank at the PSD increased to ~150% of controls while the median distance of label from postsynaptic membrane increased by 7.5 nm. These results indicate a preferential recruitment of Shank to more distal parts of the PSD complex. Conversely, upon incubation in Ca2+-free medium containing EGTA, the labeling intensity of Shank at the PSD decreased to ~70% ...Continue Reading

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Jun 22, 2014·Biochemical and Biophysical Research Communications·Jung-Hwa Tao-ChengAyse Dosemeci

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Citations

Feb 18, 2016·Molecular Neurobiology·Daniela VallejoNibaldo C Inestrosa
Sep 12, 2015·PloS One·Ayse DosemeciJung-Hwa Tao-Cheng
Sep 7, 2016·Frontiers in Synaptic Neuroscience·Ayse DosemeciJung-Hwa Tao-Cheng
Oct 17, 2018·PloS One·Ayse DosemeciThomas S Reese
May 6, 2016·PloS One·Jung-Hwa Tao-ChengAyse Dosemeci
Jan 30, 2018·F1000Research·Martijn SeltenNael Nadif Kasri
Feb 10, 2017·Nature Reviews. Neuroscience·Patricia Monteiro, Guoping Feng
Nov 1, 2016·Neural Plasticity·Tasnuva Sarowar, Andreas M Grabrucker
Dec 29, 2017·PloS One·Ayse DosemeciJung-Hwa Tao-Cheng
Apr 3, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yoonju KimKea Joo Lee
Mar 19, 2021·Biochemistry and Biophysics Reports·Erik I HallinPetri Kursula
May 20, 2021·Life Science Alliance·Tatsuo SuzukiKatsuhiko Tabuchi

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Methods Mentioned

BETA
electron microscopy
fluorescence microscopy

Software Mentioned

Synergy
KaleidaGraph

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