Differential effect of Androctonus australis hector venom components on macrophage KV channels: electrophysiological characterization

European Biophysics Journal : EBJ
Dalila KhemiliDjelila Hammoudi-Triki

Abstract

Neurotoxins of scorpion venoms modulate ion channels. Voltage-gated potassium (KV) channels regulate the membrane potential and are involved in the activation and proliferation of immune cells. Macrophages are key components of the inflammatory response induced by scorpion venom. The present study was undertaken to investigate the effect of Androctonus australis hector (Aah) venom on KV channels in murine resident peritoneal macrophages. The cytotoxicity of the venom was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) -based assay and electrophysiological recordings were performed using the whole-cell patch clamp technique. High doses of Aah venom (50, 125, 250 and 500 µg/ml) significantly decreased cell viability, while concentrations of 0.1-25 µg/ml were not cytotoxic towards peritoneal macrophages. Electrophysiological data revealed a differential block of KV current between resting and LPS-activated macrophages. Aah venom significantly reduced KV current amplitude by 62.5 ± 4.78% (n = 8, p < 0.05), reduced the use-dependent decay of the current, decreased the degree of inactivation and decelerated the inactivation process of KV current in LPS-activated macrophages. Unlike cloned KV1.5 cha...Continue Reading

References

Jan 1, 1989·Biophysical Journal·S Grissmer, M Cahalan
Apr 1, 1989·The Journal of General Physiology·S Grissmer, M D Cahalan
Jul 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·M Ismail
Jul 10, 1999·Toxicon : Official Journal of the International Society on Toxinology·M M MagalhãesJ R Cunha-Melo
Aug 17, 1999·European Journal of Pharmacology·I M MatosM M Teixeira
Apr 19, 2000·Journal of Leukocyte Biology·C M BorgesM M Teixeira
Jan 4, 2001·Toxicon : Official Journal of the International Society on Toxinology·I M De-MatosM M Teixeira
Dec 7, 2002·Toxicon : Official Journal of the International Society on Toxinology·Y D M FukuharaE A Donadi
Mar 27, 2003·Toxicon : Official Journal of the International Society on Toxinology·Daniela Trinca BertazziEliane C Arantes
Aug 19, 2003·The Journal of Biological Chemistry·Rubén VicenteAntonio Felipe
Dec 17, 2003·The Biochemical Journal·Stéphanie MouhatJean-Marc Sabatier
Mar 31, 2004·Transactions of the Royal Society of Tropical Medicine and Hygiene·Djélila Hammoudi-TrikiFatima Laraba-Djebari
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Heike WulffK George Chandy
Mar 17, 2005·Mediators of Inflammation·Vera L Petricevich, Ivo Lebrun
Mar 15, 2006·Nature Structural & Molecular Biology·Julio F Cordero-MoralesEduardo Perozo
Mar 15, 2006·Nature Structural & Molecular Biology·Julio F Cordero-MoralesEduardo Perozo
Oct 14, 2006·The Journal of Biological Chemistry·Rubén VicenteAntonio Felipe
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael D CarrithersStephen G Waxman
Oct 9, 2007·Nature Structural & Molecular Biology·Julio F Cordero-MoralesEduardo Perozo
Jun 4, 2008·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Sonia Adi-BessalemFatima Laraba-Djebari
Jan 13, 2009·The Journal of Biological Chemistry·Michael D CarrithersStephen G Waxman
Sep 17, 2009·Immunological Reviews·Michael D Cahalan, K George Chandy
Jan 27, 2010·The Journal of General Physiology·Núria VillalongaAntonio Felipe
Jun 1, 2010·Immunity·Siamon Gordon, Fernando O Martinez
Oct 26, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Adolfo BorgesJuan B De Sanctis
May 7, 2011·Inflammation·Sonia Adi-BessalemFatima Laraba-Djebari
Oct 15, 2011·Nature Reviews. Immunology·Peter J Murray, Thomas A Wynn
Jul 21, 2012·PLoS Neglected Tropical Diseases·Aouatef Ait-Lounis, Fatima Laraba-Djebari
Jul 16, 2013·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Hadjer SaidiFatima Laraba-Djebari
Nov 20, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cristina MorenoCarmen Valenzuela
Mar 4, 2014·Toxicon : Official Journal of the International Society on Toxinology·Vanesa Ramírez-BelloGina D'Suze
Jan 21, 2015·International Immunopharmacology·Imène Chaïr-YousfiDjelila Hammoudi-Triki
Apr 12, 2015·Annual Review of Immunology·Stefan FeskeEdward Y Skolnik
Apr 29, 2015·Toxicon : Official Journal of the International Society on Toxinology·Reza HadaddezfuliSamahir Saadi
Apr 30, 2015·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Nadjia BekkariFatima Laraba-Djebari
Sep 26, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Aouatef Ait-Lounis, Fatima Laraba-Djebari
Sep 30, 2015·Toxicon : Official Journal of the International Society on Toxinology·Samahir SaadiReza Hadaddezfuli
Nov 14, 2015·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Andrea Casella-MartinsLuciana S Pereira-Crott

❮ Previous
Next ❯

Citations

May 28, 2021·Frontiers in Immunology·Rachael Y M RyanJohn J Miles

❮ Previous
Next ❯

Methods Mentioned

BETA
lavage

Software Mentioned

Origin
C lampfit
p C lamp

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.