Differential effect of extracellular matrix derived from papillary and reticular fibroblasts on epidermal development in vitro

European Journal of Dermatology : EJD
David JansonAbdoelwaheb El Ghalbzouri

Abstract

Papillary and reticular fibroblasts have different effects on keratinocyte proliferation and differentiation. The aim of this study was to investigate whether these effects are caused by differential secretion of soluble factors or by differential generation of extracellular matrix from papillary and reticular fibroblasts. To study the effect of soluble factors, keratinocyte monolayer cultures were grown in papillary or reticular fibroblast-conditioned medium. To study the effect of extracellular matrix, keratinocytes were grown on papillary or reticular-derived matrix. Conditioned medium from papillary or reticular fibroblasts did not differentially affect keratinocyte viability or epidermal development. However, keratinocyte viability was increased when grown on matrix derived from papillary, compared with reticular, fibroblasts. In addition, the longevity of the epidermis was increased when cultured on papillary fibroblast-derived matrix skin equivalents compared with reticular-derived matrix skin equivalents. The findings indicate that the matrix secreted by papillary and reticular fibroblasts is the main causal factor to account for the differences in keratinocyte growth and viability observed in our study. Differences in ...Continue Reading

Citations

Jun 21, 2019·Cells·Alexandra L RippaEkaterina A Vorotelyak
Mar 20, 2018·British Journal of Cancer·Marieke HogervorstAbdoelwaheb El Ghalbzouri
Nov 17, 2019·Ageing Research Reviews·Barbara Bellei, Mauro Picardo
Feb 10, 2021·Burns : Journal of the International Society for Burn Injuries·Rajiv S RaktoeAbdoelwaheb El Ghalbzouri
May 27, 2021·Immunological Reviews·Donovan Correa-GallegosYuval Rinkevich
Aug 12, 2021·Npj Regenerative Medicine·Rita N GomesDiana S Nascimento

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