Differential effect of global ischemia on the ryanodine-sensitive and ryanodine-insensitive calcium uptake of cardiac sarcoplasmic reticulum.

Circulation Research
J J FeherN H Manson

Abstract

The effect of ischemia on the function of cardiac sarcoplasmic reticulum (SR) was assessed by the calcium uptake rate of rat whole-heart homogenates in the presence of 10 mM oxalate. Previous studies have shown that this uptake is restricted to the SR. The contribution of the ryanodine-sensitive fractions of the SR to the total homogenate uptake was assessed by using 20 microM ruthenium red and 625 microM ryanodine to close the SR calcium release channel under previously established optimal conditions. Global ischemia of 10, 15, 30, and 60 minutes depressed homogenate calcium uptake rate 19 +/- 2%, 50 +/- 6%, 65 +/- 3%, and 81 +/- 5%, respectively. This decrease was not observed when the uptake rates were measured after closure of the calcium channel with ryanodine or ruthenium red. Similar results were obtained with a Langendorff in vitro perfusion preparation, in which calcium uptake was decreased 35 +/- 5%, 37 +/- 8%, 58 +/- 7%, and 64 +/- 4% after 10, 15, 30, and 60 minutes of ischemia, but no significant decrease was observed when homogenate uptake rates were measured in the presence of ryanodine. Thus, ischemia caused a depression in the calcium uptake rate of cardiac SR only when this activity was measured in the absence...Continue Reading

References

Jun 11, 1985·Biochimica Et Biophysica Acta·B AntoniuN Ikemoto
Mar 13, 1987·Biochemical and Biophysical Research Communications·F A LaiG Meissner
Sep 1, 1987·The American Journal of Physiology·E RousseauG Meissner
Feb 1, 1988·Circulation Research·J A HillH C Strauss
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L HymelH Schindler
Feb 29, 1988·Biochemical and Biophysical Research Communications·F A LaiG Meissner
Mar 29, 1985·Biochemical and Biophysical Research Communications·L G Mészáros, N Ikemoto
Dec 1, 1988·Journal of Molecular and Cellular Cardiology·J J FeherJ L Poland
Aug 1, 1986·Journal of Molecular and Cellular Cardiology·S T RapundaloJ J Feher
Jun 30, 1987·Biochimica Et Biophysica Acta·B H Alderson, J J Feher
Apr 1, 1987·Japanese Circulation Journal·N KonnoT Katagiri
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E MarbanV P Chacko
Sep 1, 1987·The Journal of Physiology·R G Hansford, E G Lakatta
Feb 28, 1985·Biochimica Et Biophysica Acta·J J Feher, G B Lipford
Nov 1, 1988·Pflügers Archiv : European journal of physiology·J A Lee, D G Allen
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H C LeeW T Clusin
Sep 10, 1985·Biochimica Et Biophysica Acta·J J Feher, G B Lipford
Jul 31, 1985·Biochemical and Biophysical Research Communications·M B TurlaM Shlafer
Jun 1, 1971·Journal of Molecular and Cellular Cardiology·W G NaylerD Chipperfield
Jul 1, 1973·The American Journal of Cardiology·A SchwartzM Suzuki
Jan 1, 1969·Pure and Applied Chemistry. Chimie Pure Et Appliquée·S Garattini
Nov 1, 1983·Cardiovascular Research·K ImaiA Schwartz
Jul 21, 1980·Life Sciences·W R TrumbleJ P Reeves
Sep 1, 1981·Journal of Molecular and Cellular Cardiology·D O LevitskyE I Chazov
Nov 5, 1984·Nature·P H Cobbold, P K Bourne
Dec 15, 1984·The Biochemical Journal·G M BurgessJ W Putney

❮ Previous
Next ❯

Citations

Mar 1, 1991·Cardiovascular Drugs and Therapy·L H Opie
Jun 10, 1998·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·Y KumadaH Hirose
Sep 29, 1998·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·S SekiguchiT Takaba
Sep 1, 1995·Journal of Molecular and Cellular Cardiology·R W Behling, H J Malone
Aug 10, 1999·European Journal of Pharmacology·T TangA K Ho
Apr 24, 2001·Pharmacology & Therapeutics·R ZucchiS Ronca-Testoni
Nov 4, 2000·The Biochemical Journal·A BandyopadhyayD H Kim
Aug 1, 1993·The Journal of Clinical Investigation·T TakasagoH Suga
Jan 1, 1993·Archives Internationales De Physiologie, De Biochimie Et De Biophysique·E A Aiello, A O Grassi de Gende
Sep 10, 2009·Journal of Cardiovascular Pharmacology·Sabina FrascarelliRiccardo Zucchi
Mar 21, 2009·Journal of Cellular and Molecular Medicine·Sandra GhelardoniRiccardo Zucchi
Jul 7, 2001·American Journal of Physiology. Endocrinology and Metabolism·R TuplingN McKee
Aug 13, 1999·The American Journal of Physiology·R M TemsahN S Dhalla
Dec 1, 1996·The American Journal of Physiology·H J GreenJ C Dossett-Mercer

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