PMID: 8602362Feb 1, 1996Paper

Differential effect of H1 variant overexpression on cell cycle progression and gene expression

Nucleic Acids Research
D T BrownD B Sittman

Abstract

To identify functional differences among non-allelic variants of the mammalian H1 linker histones a system for the overexpression of individual H1 variants in vivo was developed. Mouse 3T3 cells were transformed with an expression vector containing the coding regions for the H1c or H10 variant under the control of an inducible promoter. Stable, single colony transformants, in which the normal stoichiometry of H1 variants was perturbed, displayed normal viability, unaltered morphology and no long-term growth arrest. However, upon release from synchronization at different points in the cell cycle transformants significantly overproducing H10 exhibited transient inhibition of both G1 and S phase progression. Overexpression of H1c to comparable levels had no effect on cell cycle progression. Analysis of transcript levels for several cell cycle-regulated and housekeeping genes indicated that overexpression of H10 resulted in significantly reduced expression of all genes tested. Surprisingly, overexpression of H1c to comparable levels resulted in either a negligible effect or, in some cases, a dramatic increase in transcript levels. These results support the suggestion that functional differences exist among H1 variants.

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F KeppelH Eisen
Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·K M NewrockL H Cohen
Jan 25, 1977·Journal of Molecular Biology·M Noll, R D Kornberg
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H KiyokawaP A Marks
Dec 1, 1992·Genes & Development·G E CrostonJ T Kadonaga
Jun 1, 1992·Trends in Biochemical Sciences·D L Poccia, G R Green
Jan 1, 1992·Molecular and Cellular Biology·C C Chipev, A P Wolffe
Jan 1, 1991·Annual Review of Biochemistry·H R Herschman
Mar 1, 1990·Molecular and Cellular Biology·J L Feng, B Villeponteau
Jul 11, 1990·Nucleic Acids Research·K W Hasel, J G Sutcliffe
Dec 1, 1989·Molecular and Cellular Biology·A ShimamuraA Worcel
Jan 1, 1989·Nucleic Acids Research·D Wells, C McBride
Nov 3, 1989·Science·A B Pardee
Jul 7, 1989·Science·J M SunA Ruiz-Carrillo
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G H ChengA I Skoultchi
Jun 1, 1988·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·R W Lennox, L H Cohen
Oct 1, 1987·International Journal of Peptide and Protein Research·R D Cole
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T A Chen, V G Allfrey
Dec 4, 1969·Biochemical and Biophysical Research Communications·S Panyim, R Chalkley
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G W StuartR D Palmiter
Nov 1, 1983·Molecular and Cellular Biology·M F LawP M Howley
Dec 1, 1981·Experimental Cell Research·S M SeyedinW S Kistler
Nov 25, 1981·Nucleic Acids Research·D L Bates, J O Thomas
May 1, 1980·Nature·J Pehrson, R D Cole
Dec 25, 1980·Nature·J AllanF X Aviles
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·A M SirotkinA I Skoultchi
Apr 1, 1994·Journal of Cellular Biochemistry·D DoeneckeB Drabent
Apr 25, 1994·Nucleic Acids Research·Y DongA I Skoultchi

❮ Previous
Next ❯

Citations

Oct 18, 2013·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Yue-Qiang ZhaoVictoria V Lunyak
Mar 30, 2013·Trends in Genetics : TIG·Kiran Mahajan, Nupam P Mahajan
Feb 1, 1997·Molecular and Biochemical Parasitology·T M NollN J Fasel
Jul 10, 1998·Biochimica Et Biophysica Acta·K FrankeD Doenecke
May 17, 2013·Journal of Proteome Research·Kelly H TeluAmir Mortazavi
Nov 5, 1997·DNA and Cell Biology·T MeergansD Doenecke
Aug 16, 2013·Nucleic Acids Research·Sean W HarshmanMichael A Freitas
Jun 17, 2003·Molecular and Cellular Biology·Yuhong FanArthur I Skoultchi
Nov 13, 2003·Molecular and Cellular Biology·Alejandro ContrerasRafael E Herrera
Jul 27, 2001·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·P T Georgel, J C Hansen
Aug 5, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David T Brown
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Missag H Parseghian, Keith A Luhrs
May 24, 2007·The Journal of Reproduction and Development·Masataka FuruyaYasunori Yoshimura
Jan 23, 2008·Biological Chemistry·Annalisa IzzoRobert Schneider
Dec 29, 2011·Frontiers in Bioscience (Landmark Edition)·Magdalena MedrzyckiYuhong Fan
Nov 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Dhananjay Yellajoshyula, David T Brown
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Raouf AlamiEric E Bouhassira
Jul 27, 2001·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M H Parseghian, B A Hamkalo
May 20, 2008·Animal Reproduction Science·M C O CavalcantiM Bergmann
Oct 11, 2015·Biochimica Et Biophysica Acta·Thomas W Flanagan, David T Brown
Jul 13, 2005·The FEBS Journal·Bettina SargHerbert H Lindner
Apr 27, 2007·Annals of the New York Academy of Sciences·Kalliope E Sekeri-Pataryas, Thomae G Sourlingas
Oct 19, 2012·Cell Biology International·Andrzej Kowalski, Jan Pałyga
Jul 17, 2009·Molecular Reproduction and Development·Margaret L ShawRichard Saffery
Jun 30, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mohammadi KaouassDanuta Balicki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.