Differential effect of hypoxia on human mesenchymal stem cell chondrogenesis and hypertrophy in hyaluronic acid hydrogels

Acta Biomaterialia
Meiling ZhuLiming Bian

Abstract

Photocrosslinked hyaluronic acid (HA) hydrogels provide a conducive 3-D environment that supports the chondrogenesis of human mesenchymal stem cells (hMSCs). The HA macromer concentration in the hydrogels has a significant impact on the chondrogenesis of the encapsulated MSCs due to changes in the physical properties of the hydrogels. Meanwhile, hypoxia has been shown to promote MSC chondrogenesis and suppress subsequent hypertrophy. This study investigates the combinatorial effect of tuning HA macromer concentration (1.5-5%w/v) and hypoxia on MSC chondrogenesis and hypertrophy. To decouple the effect of HA concentration from that of crosslinking density, the HA hydrogel crosslinking density was adjusted by varying the extent of the reaction through the light exposure time while keeping the HA concentration constant (5%w/v at 5 or 15 min). It was found that hypoxia had no significant effect on the chondrogenesis and cartilaginous matrix synthesis of hMSCs under all hydrogel conditions. In contrast, the hypoxia-mediated positive or negative regulation of hMSC hypertrophy in HA hydrogels is dependent on the HA concentration but independent of the crosslinking density. Specifically, hypoxia significantly suppressed hMSC hypertroph...Continue Reading

References

May 1, 1984·Calcified Tissue International·C C ChenL C Rosenberg
Apr 1, 1994·The Journal of Clinical Investigation·A P HollanderA R Poole
Nov 15, 2000·Journal of Biomedical Materials Research·K A SmedsM W Grinstaff
Jun 6, 2003·The Journal of Biological Chemistry·Maija HirsiläJohanna Myllyharju
Oct 29, 2003·Current Opinion in Biotechnology·Jeffrey A Hubbell
Apr 23, 2004·Trends in Cell Biology·Nathalie OrtegaZena Werb
Feb 17, 2006·Journal of Biomedical Materials Research. Part a·Cindy ChungJason A Burdick
Aug 15, 2006·The Journal of Biological Chemistry·Makoto HiraoAkira Myoui
Sep 7, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Maria A Mello, Rocky S Tuan
May 2, 2007·The Journal of Cell Biology·Sylvain ProvotErnestina Schipani
Apr 29, 2008·Arthritis and Rheumatism·Michael B Mueller, Rocky S Tuan
Sep 2, 2008·Arthritis and Rheumatism·Brian JubeckAnn K Rosenthal
Aug 29, 2009·Arthritis and Rheumatism·Brian JubeckAnn K Rosenthal
Dec 8, 2009·Science·Richard O Hynes
Apr 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Celeste ScottiIvan Martin
Dec 14, 2011·Biochemical and Biophysical Research Communications·Eamon J SheehyDaniel J Kelly
Mar 2, 2012·Journal of Cellular Physiology·Nalinkanth V Ghone, Warren L Grayson
May 9, 2012·Tissue Engineering. Part a·Debby GawlittaWouter J A Dhert
Jun 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Liming BianJason A Burdick

❮ Previous
Next ❯

Citations

Jun 28, 2014·International Orthopaedics·Liliana S Moreira TeixeiraFrank P Luyten
Jan 31, 2016·Biomechanics and Modeling in Mechanobiology·Adam OReillyDaniel J Kelly
Nov 26, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Adam O'Reilly, Daniel J Kelly
Feb 22, 2017·Journal of Biomedical Materials Research. Part a·Joaquin Rodenas-RochinaMyriam Lebourg
May 26, 2017·Cellular & Molecular Biology Letters·Ewelina Aleksander-KonertAdam Wilczok
Jan 27, 2019·International Journal of Molecular Sciences·Girish PattappaPeter Angele

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.