Differential effect of intestinal neuropeptides on invasion and migration of colon carcinoma cells in vitro

Cancer Letters
M OgasawaraI Saimi

Abstract

We investigated the effect of neuropeptides, which are vasoactive intestinal polypeptide (VIP), substance P, (SP), neuropeptide Y (NPY), neurokinin A (NKA), somatostatin (SOM), calcitonin gene-related peptide (CGRP), and leucine-enkephalin (L-ENK), on the invasion of murine Colon 26-L5 adenocarcinoma cells through a reconstituted basement membrane (Matrigel) using a Transwell cell culture chamber assay. VIP, SP, NPY, and L-ENK reduced invasive potential of tumor cells in a concentration-dependent manner, whereas SOM, CGRP, and NKA had no effect. Especially, VIP showed the most effective in inhibiting tumor invasion, and achieved 50% reduction at 10(-6) M. A similar effect by VIP was also observed in cell migration to fibronectin. VIP had no effect on the growth of tumor cells at the concentrations ranging from 10(-10) to 10(-6) M. The suppressed ability of the tumor cell motility by VIP (10(-6) M) was practically recovered by co-treatment with 2',5'-dideoxyadenosine, an adenylate cyclase inhibitor. These results indicate that VIP, among the neuropeptides used, could inhibit Matrigel invasion of Colon 26-L5 carcinoma cells through partial suppression of their motility, and the reduction was associated with an intracellular cAMP-...Continue Reading

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Citations

Dec 23, 2003·The International Journal of Biochemistry & Cell Biology·Petr BusekAleksi Sedo
Mar 10, 2001·International Journal of Urology : Official Journal of the Japanese Urological Association·O NagakawaI Saiki
Jul 2, 2005·Neuroimmunomodulation·Jill M Manske, Summer E Hanson
Jan 31, 2016·Peptides·Madryssa de BoisvilliersCorinne Chadéneau
Sep 6, 2006·Gastroenterology·Rodger A Liddle, Randy L Jirtle
May 15, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Helmut FriessMarkus W Büchler
Jan 31, 2021·Nature Reviews. Gastroenterology & Hepatology·Beate NieslerKarl-Herbert Schäfer

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