PMID: 9430821Feb 7, 1998Paper

Differential effect of keratinocyte growth factor (KGF) on aromatase activity in cultured canine prostatic epithelial cells

Endocrine Research
H CanatanY C Lin

Abstract

We have recently cloned the mRNA encoding KGF from canine prostate and produced recombinant canine KGF (rcKGF) which specifically acts on cultured canine prostatic epithelial cells (CCPECs) which possess KGF receptors (Canatan et al., 1996; DNA Cell Biol. 15:247). In the present study, the effect of rcKGF on aromatase activity in CCPECs from young (6-month-old) and mature (3-year-old) dogs was examined. Release of 3H2O from labeled substrate was used as the indicator of aromatase activity. CCPECs were pulsed with [1-beta-3H]-androstenedione (1 microCi/ml, 6 hr). The amounts of 3H2O released into culture medium were measured (dpm) and total cellular proteins were determined. Aromatase activity was expressed as 3H2O dpm/mg cellular protein (mean +/- SEM). The basal level of aromatase activity in CCPECs from mature dogs was approximately 4 times higher (p < 0.05) than that in cells from young dogs. Aromatase activity in CCPECs from mature dogs increased in a dose-dependent manner upon treatment with rcKGF. Interestingly, rcKGF, at any of the concentrations tested, had no significant effect on aromatase activity in CCPECs from young dogs. These results are the first to indicate that aromatase activity is affected by KGF in mature C...Continue Reading

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Citations

Nov 25, 2000·DNA Sequence : the Journal of DNA Sequencing and Mapping·H Canatan, Y C Lin
Jul 21, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Yi-Wen HuangYoung C Lin
Nov 30, 2007·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·F Shidaifat
Jul 13, 2002·Annals of the New York Academy of Sciences·M HerrmannR H Straub

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