Differential Effect of Newly Isolated Phages Belonging to PB1-Like, phiKZ-Like and LUZ24-Like Viruses against Multi-Drug Resistant Pseudomonas aeruginosa under Varying Growth Conditions

Viruses
Simone LatzHans-Peter Horz

Abstract

In this study, we characterize three phages (SL1 SL2, and SL4), isolated from hospital sewage with lytic activity against clinical isolates of multi-drug resistant Pseudomonas aeruginosa (MDR-PA). The host spectrum ranged from 41% to 54%, with all three phages together covering 79% of all tested clinical isolates. Genome analysis revealed that SL1 (65,849 bp, 91 open reading frames ORFs) belongs to PB1-like viruses, SL2 (279,696 bp, 354 ORFs) to phiKZ-like viruses and SL4 (44,194 bp, 65 ORFs) to LUZ24-like viruses. Planktonic cells of four of five selected MDR-PA strains were suppressed by at least one phage with multiplicities of infection (MOIs) ranging from 1 to 10-6 for 16 h without apparent regrowth of bacterial populations. While SL2 was most potent in suppressing planktonic cultures the strongest anti-biofilm activity was observed with SL4. Phages were able to rescue bacteria-infected wax moth larvae (Galleria melonella) for 24 h, whereby highest survival rates (90%) were observed with SL1. Except for the biofilm experiments, the effect of a cocktail with all three phages was comparable to the action of the best phage alone; hence, there are no synergistic but also no antagonistic effects among phages. The use of a cockt...Continue Reading

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Citations

Mar 30, 2018·Viruses·Irene HuberWolfgang Beyer
Nov 19, 2019·Frontiers in Microbiology·Cátia PacíficoFriederike Hilbert
Dec 19, 2019·Frontiers in Microbiology·Mohammed ImamEdouard E Galyov
Sep 27, 2020·Viruses·Maria L SokolovaKonstantin V Severinov
Oct 2, 2020·Annals of Clinical Microbiology and Antimicrobials·Zahra CheginiAref Shariati
Jan 28, 2021·Viruses·Victor KrylovVitaly Zverev
Jan 29, 2021·Infection and Drug Resistance·Fengjuan TianYigang Tong
Jul 3, 2021·International Journal of Molecular Sciences·Ho Yin Pekkle LamKai-Chih Chang

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Datasets Mentioned

BETA
MF768469

Methods Mentioned

BETA
PCR
Assay
transmission electron microscopy
Infection

Software Mentioned

BLASTn
PHAST
IMAGE SP Professional
SE
BLASTx
GeneMark
GelQuest
tRNAscan
hmm

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